CRISPR-Cas12a-based genome editing and transcriptional repression for biotin synthesis in Pseudomonas mutabilis.
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DOI: 10.1093/jambio/lxad049
Abstract: AIMS To establish a dual-function CRISPR-Cas12a system combined genome editing and transcriptional repression for multiplex metabolic engineering of Pseudomonas mutabilis. MATERIALS AND RESULTS This CRISPR-Cas12a system consisted of two plasmids that enabled single gene deletion,… read more here.
Keywords: genome editing; editing transcriptional; mutabilis; repression ... See more keywords