Amplification Efficiency and Template Accessibility as Distinct Causes of Rain in Digital PCR: Monte Carlo Modeling and Experimental Validation.
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DOI: 10.1021/acs.analchem.2c03534
Abstract: Partitions in digital PCR (dPCR) assays do not reach the detection threshold at the same time. This heterogeneity in amplification results in intermediate endpoint fluorescence values (i.e., rain) and misclassification of partitions, which has a… read more here.
Keywords: template accessibility; amplification efficiency; rain; efficiency template ... See more keywords