Efficient base editing for multiple genes and loci in pigs using base editors
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10421-8
Abstract: Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-stranded breaks and homology-directed repair in a variety of organisms, which exhibit great potential for agricultural and biomedical applications. However, all reported cases only involved… read more here.
Keywords: base editing; multiple sites; efficient base; editing multiple ... See more keywords
An efficient base and H2O2 free protocol for the synthesis of phenols in water and oxygen using spinel CuFe2O4 magnetic nanoparticles
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Coordination Chemistry"
DOI: 10.1080/00958972.2020.1802437
Abstract: Abstract An efficient base and H2O2 free protocol was used for the synthesis of phenols from boronic acids using biogenic CuFe2O4 magnetic nanoparticles as catalyst at room temperature in water and oxygen. The catalyst was… read more here.
Keywords: base h2o2; efficient base; h2o2 free; protocol ... See more keywords
Highly efficient C‐to‐T and A‐to‐G base editing in a Populus hybrid
Sign Up to like & getrecommendations! Published in 2021 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.13581
Abstract: CRISPR-Cas based genome editing technologies hold great potential for genetic research and bioengineering in trees. CRISPR-Cas9 was previously demonstrated to be a highly efficient genome editing system in poplar (Zhou et al., 2015). However, these… read more here.
Keywords: base editing; efficient base; base; editing populus ... See more keywords
Efficient Base-Catalyzed Kemp Elimination in an Engineered Ancestral Enzyme
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23168934
Abstract: The routine generation of enzymes with completely new active sites is one of the major unsolved problems in protein engineering. Advances in this field have been so far modest, perhaps due, at least in part,… read more here.
Keywords: efficient base; kemp elimination; engineering; elimination ... See more keywords