Highly efficient CRISPR systems for loss-of-function and gain-of-function research in pear calli
Sign Up to like & getrecommendations! Published in 2022 at "Horticulture Research"
DOI: 10.1093/hr/uhac148
Abstract: Abstract CRISPR/Cas systems have been widely used for genome engineering in many plant species. However, their potentials have remained largely untapped in fruit crops, particularly in pear, due to the high levels of genomic heterozygosity… read more here.
Keywords: function; loss function; crispr; gain function ... See more keywords
An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
Sign Up to like & getrecommendations! Published in 2019 at "eLife"
DOI: 10.7554/elife.51539
Abstract: We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic library for multiple uses (Lee et al., 2018a). The method relied on double stranded DNA (dsDNA) homology donors with… read more here.
Keywords: homology arms; crispr based; based strategy; efficient crispr ... See more keywords