Implementing CRISPR-Cas12a for Efficient Genome Editing in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_7
Abstract: The oleaginous yeast Yarrowia lipolytica has emerged as an industrially relevant chassis to produce various valuable chemicals. Metabolic engineering of Y. lipolytica relies on the availability of genetic engineering tools. Existing engineering strategies for this yeast… read more here.
Keywords: genome editing; crispr cas12a; efficient genome; lipolytica ... See more keywords
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method
Sign Up to like & getrecommendations! Published in 2022 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2022.02.002
Abstract: Claviceps purpurea produces many pharmacologically important ergot alkaloids (EAS), which are widely used to treat migraine and hypertension and to aid childbirth. Although an EAS biosynthetic cluster of C. purpurea has been discovered more than… read more here.
Keywords: crispr cas9; efficient genome; ribonucleoprotein; genome editing ... See more keywords
Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing
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DOI: 10.1021/acschembio.2c00116
Abstract: CRISPR-Cas technology has revolutionized genome editing. Its broad and fast-growing application in biomedical research and therapeutics has led to increased demand for guide RNAs. The synthesis of chemically modified single-guide RNAs (sgRNAs) containing >100 nucleotides… read more here.
Keywords: genome editing; efficient genome; ligated crispr; tetrazine ligated ... See more keywords
Repriming DNA synthesis: an intrinsic restart pathway that maintains efficient genome replication
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DOI: 10.1093/nar/gkab176
Abstract: Abstract To bypass a diverse range of fork stalling impediments encountered during genome replication, cells possess a variety of DNA damage tolerance (DDT) mechanisms including translesion synthesis, template switching, and fork reversal. These pathways function… read more here.
Keywords: dna synthesis; restart pathway; genome replication; efficient genome ... See more keywords
Wristwatch PCR: A Versatile and Efficient Genome Walking Strategy
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DOI: 10.3389/fbioe.2022.792848
Abstract: Genome walking is a method used to retrieve unknown flanking DNA. Here, we reported wristwatch (WW) PCR, an efficient genome walking technique mediated by WW primers (WWPs). WWPs feature 5′- and 3′-overlap and a heterologous… read more here.
Keywords: pcr versatile; wristwatch pcr; genome walking; efficient genome ... See more keywords