A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either parameter
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DOI: 10.1002/biot.202000340
Abstract: Incubation at pH 4.0 or blanching at ∼65°C facilitates the purification of biopharmaceutical proteins from plants by precipitating most of the host cell proteins (HCPs) before chromatography. However, both methods are compatible only with pH… read more here.
Keywords: tobacco; temperature; facilitates purification; recombinant proteins ... See more keywords