Employing intercalated redox reporter into electrochemical aptamer-based biosensors to enable calibration-free molecular measurements in undiluted serum.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c02205
Abstract: Electrochemical aptamer-based (E-AB) biosensor suffers from sensor-to-sensor signal variation due to the variation of total number of probes immobilized on sensor surface and heterogeneity of probe distributions, with the former attracting more attention. As such,… read more here.
Keywords: electrochemical aptamer; aptamer based; redox reporter; calibration free ... See more keywords
Finding the Lost Dissociation Constant of Electrochemical Aptamer-Based Biosensors.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03566
Abstract: Electrochemical aptamer-based (E-AB) biosensors afford real-time measurements of the concentrations of molecules directly in complex matrices and in the body, offering alternative strategies to develop innovative personalized medicine tools. While different electroanalytical techniques have been… read more here.
Keywords: dissociation; aptamer based; based biosensors; spectroscopy ... See more keywords
Incorporating Hydrophobic Moieties into Self-Assembled Monolayers to Enable Electrochemical Aptamer-Based Sensors Deployed Directly in a Complex Matrix.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c00995
Abstract: Continuous real-time measurement of specific targets in complex biological samples is of great significance for early diagnosis and treatment of diseases and thus enables achievement of personalized medicine. Electrochemical aptamer-based (E-AB) sensors are good candidates… read more here.
Keywords: aptamer based; complex matrix; electrochemical aptamer; assembled monolayers ... See more keywords
Optimization of Vancomycin Aptamer Sequence Length Increases the Sensitivity of Electrochemical, Aptamer-Based Sensors In Vivo.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c01910
Abstract: The measurement of serum vancomycin levels at the clinic is critical to optimizing dosing given the narrow therapeutic window of this antibiotic. Current approaches to quantitate serum vancomycin levels are based on immunoassays, which are… read more here.
Keywords: aptamer based; vancomycin; electrochemical aptamer; sequence ... See more keywords
Week-Long Operation of Electrochemical Aptamer Sensors: New Insights into Self-Assembled Monolayer Degradation Mechanisms and Solutions for Stability in Serum at Body Temperature.
Sign Up to like & getrecommendations! Published in 2023 at "ACS sensors"
DOI: 10.1021/acssensors.2c02403
Abstract: Conventional wisdom suggests that widely utilized self-assembled alkylthiolate monolayers on gold are too unstable to last more than several days when exposed to complex fluids such as raw serum at body temperature. Demonstrated here is… read more here.
Keywords: self assembled; electrochemical aptamer; monolayer degradation; aptamer ... See more keywords
Switching the aptamer attachment geometry can dramatically alter the signalling and performance of electrochemical aptamer-based sensors.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical communications"
DOI: 10.1039/d1cc04557a
Abstract: Electrochemical aptamer-based (EAB) sensors, composed of an electrode-bound DNA aptamer with a redox reporter on the distal end, offer the promise of high-frequency, real-time molecular measurements in complex sample matrices and even in vivo. Here… read more here.
Keywords: switching aptamer; electrochemical aptamer; aptamer based; geometry ... See more keywords
Solution-Phase Electrochemical Aptamer-Based Sensors
Sign Up to like & getrecommendations! Published in 2022 at "IEEE Transactions on Biomedical Engineering"
DOI: 10.1109/tbme.2022.3203026
Abstract: Electrochemical aptamer-based sensors (EABs) using self-assembled monolayers on gold working-electrodes have now been in-vivo demonstrated for multiple-analytes, demonstrating their sensitivity and specificity even in a continuous sensing format. However, longevity has been demonstrated for only… read more here.
Keywords: aptamer based; electrochemical aptamer; solution phase; based sensors ... See more keywords