Structural proteomics, electron cryo-microscopy and structural modeling approaches in bacteria–human protein interactions
Sign Up to like & getrecommendations! Published in 2020 at "Medical Microbiology and Immunology"
DOI: 10.1007/s00430-020-00663-5
Abstract: A central challenge in infection medicine is to determine the structure and function of host–pathogen protein–protein interactions to understand how these interactions facilitate bacterial adhesion, dissemination and survival. In this review, we focus on proteomics,… read more here.
Keywords: protein; microscopy; structural modeling; electron cryo ... See more keywords
Electron Cryo-microscopy Structure of Ebola Virus Nucleoprotein Reveals a Mechanism for Nucleocapsid-like Assembly
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.02.009
Abstract: Ebola virus nucleoprotein (eNP) assembles into higher-ordered structures that form the viral nucleocapsid (NC) and serve as the scaffold for viral RNA synthesis. However, molecular insights into the NC assembly process are lacking. Using a hybrid… read more here.
Keywords: like assembly; ebola virus; microscopy; virus nucleoprotein ... See more keywords
Marker-free method for accurate alignment between correlated light, cryo-light, and electron cryo-microscopy data using sample support features.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2017.11.001
Abstract: Combining fluorescence microscopy with electron cryo-tomography allows, in principle, spatial localization of tagged macromolecular assemblies and structural features within the cellular environment. To allow precise localization and scale integration between the two disparate imaging modalities,… read more here.
Keywords: microscopy; accurate alignment; method; electron cryo ... See more keywords
Electron cryo-tomography of vestibular hair-cell stereocilia.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2019.02.006
Abstract: High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a… read more here.
Keywords: hair cell; cell stereocilia; cryo tomography; electron cryo ... See more keywords
Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy
Sign Up to like & getrecommendations! Published in 2021 at "Quarterly Reviews of Biophysics"
DOI: 10.1017/s0033583521000044
Abstract: Abstract Over the past decade, the structural biology of membrane proteins (MPs) has taken a new turn thanks to epoch-making technical progress in single-particle electron cryo-microscopy (cryo-EM) as well as to improvements in sample preparation.… read more here.
Keywords: particle electron; microscopy; cryo; electron cryo ... See more keywords
Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-13390-4
Abstract: The Pithoviridae giant virus family exhibits the largest viral particle known so far, a prolate spheroid up to 2.5 μm in length and 0.9 μm in diameter. These particles show significant variations in size. Little is known… read more here.
Keywords: microscopy; energy filtered; particle; electron cryo ... See more keywords
Concluding remarks: Challenges and future developments in biological electron cryo-microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Faraday discussions"
DOI: 10.1039/d2fd90062a
Abstract: During the past 10 years, biological electron cryo-microscopy (cryoEM) has undergone a process of rapid transformation. Many things we could only dream about a decade ago have now become almost routine. Nevertheless, a number of… read more here.
Keywords: microscopy; electron cryo; cryo microscopy; concluding remarks ... See more keywords
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.02.28.482229
Abstract: We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised… read more here.
Keywords: cryo tomography; cryo; electron cryo; single particle ... See more keywords
Analysis of Cell–Cell Bridges in Haloferax volcanii Using Electron Cryo-Tomography Reveal a Continuous Cytoplasm and S-Layer
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2020.612239
Abstract: Halophilic archaea have been proposed to exchange DNA and proteins using a fusion-based mating mechanism. Scanning electron microscopy previously suggested that mating involves an intermediate state, where cells are connected by an intercellular bridge. To… read more here.
Keywords: continuous cytoplasm; microscopy; layer; cryo tomography ... See more keywords