Rapid tilt-series acquisition for electron cryotomography.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2018.12.008
Abstract: Using a new Titan Krios stage equipped with a single-axis holder, we developed two methods to accelerate the collection of tilt-series. We demonstrate a continuous-tilting method that can record a tilt-series in seconds, but with… read more here.
Keywords: rapid tilt; electron cryotomography; series; biology ... See more keywords
In vivo structure of the Legionella type II secretion system by electron cryotomography
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DOI: 10.1038/s41564-019-0603-6
Abstract: The type II secretion system (T2SS) is a multiprotein envelope-spanning assembly that translocates a wide range of virulence factors, enzymes and effectors through the outer membrane of many Gram-negative bacteria1–3. Here, using electron cryotomography and… read more here.
Keywords: electron cryotomography; structure; type; vivo structure ... See more keywords