Cryo‐electron tomography—the cell biology that came in from the cold
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12757
Abstract: Cryo‐electron tomography (cryo‐ET) provides high‐resolution 3D views into cells pristinely preserved by vitrification. Recent technical advances such as direct electron detectors, the Volta phase plate and cryo‐focused ion beam milling have dramatically pushed image quality… read more here.
Keywords: cryo electron; cell biology; biology; electron tomography ... See more keywords
Correlative Laboratory Nano‐CT and 360° Electron Tomography of Macropore Structures in Hierarchical Zeolites
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Materials Interfaces"
DOI: 10.1002/admi.202001154
Abstract: Hierarchical pore structures exhibit morphological features on several length scales, which govern important materials properties in catalysis, such as catalytic activity, diffusivity or selectivity. Correlative tomography offers unique opportunities for a comprehensive and scale‐bridging 3D… read more here.
Keywords: 360 electron; nano; tomography; electron tomography ... See more keywords
Cryo‐Electron Tomography of Toxoplasma gondii Indicates That the Conoid Fiber May Be Derived from Microtubules
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Science"
DOI: 10.1002/advs.202206595
Abstract: Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis and can infect numerous warm‐blooded animals. An improved understanding of the fine structure of this parasite can help elucidate its replication mechanism. Previous studies have… read more here.
Keywords: gondii; conoid fiber; toxoplasma gondii; electron tomography ... See more keywords
Evaluation of electron tomography reconstruction methods for interface roughness measurement
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.23006
Abstract: We evaluate the suitability of simultaneous iterative reconstruction technique (SIRT), filtered back projection, and simultaneous algebraic reconstruction technique methods for buried interface roughness measurements. We also investigate the effect of total electron dose distributed over… read more here.
Keywords: reconstruction; evaluation electron; roughness measurement; electron tomography ... See more keywords
Electron Tomography Reveals the Droplet Covered Surface Structure of Nanowires Grown by Aerotaxy.
Sign Up to like & getrecommendations! Published in 2018 at "Small"
DOI: 10.1002/smll.201801285
Abstract: For the purpose of functionalizing III-V semiconductor nanowires using n-doping, Sn-doped GaAs zincblende nanowires are produced, using the growth method of Aerotaxy. The growth conditions used are such that Ga droplets, formed on the nanowire… read more here.
Keywords: growth; surface; droplet covered; electron tomography ... See more keywords
High-Resolution Electron Tomography of Ultrathin Boerdijk-Coxeter-Bernal Nanowire Enabled by Superthin Metal Surface Coating.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202203310
Abstract: The rapid advancement of transmission electron microscopy has resulted in revolutions in a variety of fields, including physics, chemistry, and materials science. With single-atom resolution, 3D information of each atom in nanoparticles is revealed, while… read more here.
Keywords: resolution; electron; metal; electron tomography ... See more keywords
Getting Started with In Situ Cryo-Electron Tomography.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0966-8_1
Abstract: Cryo-electron tomography (cryo-ET) is an extremely powerful tool which is used to image cellular features in their close-to-native environment at a resolution where both protein structure and membrane morphology can be revealed. Compared to conventional… read more here.
Keywords: cryo electron; cryo; biology; electron tomography ... See more keywords
A joint alignment and reconstruction algorithm for electron tomography to visualize in-depth cell-to-cell interactions.
Sign Up to like & getrecommendations! Published in 2022 at "Histochemistry and cell biology"
DOI: 10.1007/s00418-022-02095-z
Abstract: Electron tomography allows one to obtain 3D reconstructions visualizing a tissue's ultrastructure from a series of 2D projection images. An inherent problem with this imaging technique is that its projection images contain unwanted shifts, which… read more here.
Keywords: joint alignment; reconstruction; reconstruction algorithm; electron tomography ... See more keywords
Electron Tomography Revels that Milk Lipids Originate from Endoplasmic Reticulum Domains with Novel Structural Features
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Mammary Gland Biology and Neoplasia"
DOI: 10.1007/s10911-019-09438-y
Abstract: Lipid droplets (LD) are dynamically-regulated organelles that originate from the endoplasmic reticulum (ER), and function in the storage, trafficking and metabolism of neutral lipids. In mammary epithelial cells (MEC) of lactating animals, intact LD are… read more here.
Keywords: endoplasmic reticulum; originate endoplasmic; domains novel; lipids originate ... See more keywords
Dislocation electron tomography: A technique to characterize the dislocation microstructure evolution in zirconium alloys under irradiation
Sign Up to like & getrecommendations! Published in 2021 at "Acta Materialia"
DOI: 10.1016/j.actamat.2021.116964
Abstract: Abstract Diffraction-contrast electron tomography was used to analyse the 3D geometry of the dislocation microstructure in a zirconium alloy before and after ion irradiation. The material had been strained at room temperature prior to irradiation.… read more here.
Keywords: zirconium alloys; dislocation; electron tomography; dislocation microstructure ... See more keywords
Visualizing Infection Initiation of Bacteriophage P22 by Cryo-Electron Tomography
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1704
Abstract: For successful infection, bacteriophages must overcome multiple barriers in the bacterial cell envelope to translocate viral DNA and proteins into the host cell. However, the molecular mechanisms underlying the phage infection initiation remain poorly understood.… read more here.
Keywords: cryo electron; phage; infection; p22 ... See more keywords