Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation
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DOI: 10.1002/biot.202100325
Abstract: The increased use of biopharmaceuticals calls for improved means of bioprocess monitoring. In this work, capillary electrophoresis (CE) and microchip electrophoresis (MCE) methods were developed and applied for the analysis of amino acids (AAs) in… read more here.
Keywords: microchip electrophoresis; cultivation; analysis; amino ... See more keywords
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization
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DOI: 10.1002/btpr.3452
Abstract: Two‐dimensional electrophoresis (2DE) is a gel‐based protein separation method based on size and charge which is commonly used for the characterization of host cell proteins (HCPs) during drug development in biotech and pharmaceutical companies. HCPs… read more here.
Keywords: method; gel; gel electrophoresis; two dimensional ... See more keywords
Colloidal Fluorophore Aggregates for the Selective Detection of Albumins in Solution and on Electrophoresis Gels
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DOI: 10.1002/cbic.201800608
Abstract: We report a fluorescent probe that is highly sensitive and selective for serum albumins. Signal transduction results from the disassembly of fluorescence‐quenched aggregates upon binding to human serum albumin (HSA). The probe offers a rapid… read more here.
Keywords: fluorophore aggregates; electrophoresis; selective detection; electrophoresis gels ... See more keywords
Determination of the chiral status of different novel psychoactive substance classes by capillary electrophoresis and β‐cyclodextrin derivatives
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DOI: 10.1002/chir.23268
Abstract: Abstract Besides the abuse of well‐known illicit drugs, consumers discovered new synthetic compounds with similar effects but minor alterations in their chemical structure. Originally, these so‐called novel psychoactive substances (NPS) have been created to circumvent… read more here.
Keywords: cyclodextrin; determination chiral; electrophoresis; chiral status ... See more keywords
Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201600331
Abstract: One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biennial reviews, first published in Electrophoresis in 2007, on developments in the… read more here.
Keywords: advances enhancing; line; capillaries microchips; electrophoresis ... See more keywords
Establishment and validation of a microfluidic capillary gel electrophoresis platform method for purity analysis of therapeutic monoclonal antibodies
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DOI: 10.1002/elps.201600519
Abstract: Capillary and microfluidic chip electrophoresis technologies are heavily utilized for development, characterization, release, and stability testing of biopharmaceuticals. Within the biopharmaceutical industry, CE‐SDS and M‐CGE are commonly used for purity determination by separation and quantitation… read more here.
Keywords: platform method; method; electrophoresis; stability ... See more keywords
Separation of hydroxynorketamine stereoisomers using capillary electrophoresis with sulfated β‐cyclodextrin and highly sulfated γ‐cyclodextrin
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700016
Abstract: The racemic N‐methyl‐d‐aspartate receptor antagonist ketamine is used in anesthesia, analgesia and the treatment of depressive disorders. It is known that interactions of hydroxylated norketamine metabolites and 5,6‐dehydronorketamine (DHNK) with the α7‐nicotinic acetylcholine receptor and… read more here.
Keywords: capillary electrophoresis; ketamine; cyclodextrin highly; electrophoresis ... See more keywords
Capillary electrophoresis and nanomaterials – Part I: Capillary electrophoresis of nanomaterials
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DOI: 10.1002/elps.201700097
Abstract: Nanomaterials are in analytical science used for a broad range of purposes, covering the area of sample pretreatment as well as separation, detection, and identification of target molecules. This part of the review covers capillary… read more here.
Keywords: capillary electrophoresis; part capillary; electrophoresis; electrophoresis nanomaterials ... See more keywords
Recent applications of nanomaterials in capillary electrophoresis
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DOI: 10.1002/elps.201700178
Abstract: Nanomaterials have found an important place in Analytical Chemistry and, in particular, in Separation Science. Among them, metal‐organic frameworks, magnetic and non‐magnetic nanoparticles, carbon nanotubes and graphene, as well as their combinations, are the most… read more here.
Keywords: capillary electrophoresis; recent applications; applications nanomaterials; electrophoresis ... See more keywords
Comparison of antimicrobial peptide purification via free‐flow electrophoresis and gel filtration chromatography
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DOI: 10.1002/elps.201700187
Abstract: Antimicrobial peptides (AMPs) are usually small and cationic biomolecules with broad‐spectrum antimicrobial activities against pathogens. Purifying them from complex samples is essential to study their physiochemical properties. In this work, free‐flow zone electrophoresis (FFZE) was… read more here.
Keywords: electrophoresis; filtration chromatography; free flow; gel filtration ... See more keywords
Development of high‐throughput and high sensitivity capillary gel electrophoresis platform method for Western, Eastern, and Venezuelan equine encephalitis (WEVEE) virus like particles (VLPs) purity determination and characterization
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DOI: 10.1002/elps.201700217
Abstract: In this paper, we describe development of a high‐throughput, highly sensitive method based on Lab Chip CGE‐SDS platform for purity determination and characterization of virus‐like particle (VLP) vaccines. A capillary gel electrophoresis approach requiring about… read more here.
Keywords: method; electrophoresis; purity; platform ... See more keywords