Simulation of the influence of voltage level and pulse spacing on the efficiency, aggressiveness and uniformity of the electroporation process in tissues using meshless techniques.
Sign Up to like & getrecommendations! Published in 2020 at "International journal for numerical methods in biomedical engineering"
DOI: 10.1002/cnm.3304
Abstract: Electroporation is a widely used method consisting of application of high-voltage, short-duration electric pulses to increase cell membrane permeability, allowing cellular internalization of medications. In this work, the influence of two primary parameters, voltage level… read more here.
Keywords: electroporation; efficiency aggressiveness; voltage level; uniformity ... See more keywords
Double electroporation in two adjacent tissues in chicken embryos
Sign Up to like & getrecommendations! Published in 2018 at "Developmental Dynamics"
DOI: 10.1002/dvdy.24674
Abstract: In ovo electroporation is a well established method to introduce transgenes into a number of tissues in chicken embryos, e.g., neural tissue, limb mesenchyme, and somites. This method has been widely used to investigate cell… read more here.
Keywords: double electroporation; adjacent tissues; chicken embryos; two adjacent ... See more keywords
Bacterial inactivation via microfluidic electroporation device with insulating micropillars
Sign Up to like & getrecommendations! Published in 2021 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202000326
Abstract: Electroporation is a promising method to inactivate cells and it has wide applications in medical science, biology and environmental health. Here, we investigate the bacteria inactivation performance of two different microfluidic electroporation devices with rhombus… read more here.
Keywords: circular micropillars; field; electroporation; inactivation ... See more keywords
Deep Learning-Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard-to-Transfect Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202107795
Abstract: Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene… read more here.
Keywords: cell; electroporation; cell electroporation; manipulation ... See more keywords
Optimized siRNA Delivery into Primary Immune Cells Using Electroporation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0290-4_7
Abstract: Effective RNA delivery strategies for primary human monocytes and dendritic cells (DCs) are useful tools for both basic research and cancer immunotherapy applications. Compared to viral delivery, electroporation is a relatively safe and simple technique… read more here.
Keywords: delivery; optimized sirna; electroporation; immune cells ... See more keywords
Open the Pores: Electroporation for the Transformation of Trichoderma reesei.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_6
Abstract: During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform… read more here.
Keywords: electroporation transformation; electroporation; open pores; reesei ... See more keywords
Genome Editing in Mouse and Rat by Electroporation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7128-2_7
Abstract: Many knock-out/knock-in mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Microinjection… read more here.
Keywords: engineered endonucleases; electroporation; genome editing; mouse rat ... See more keywords
Microfluidic Device for Localized Electroporation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9740-4_10
Abstract: Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells… read more here.
Keywords: transfection; device localized; localized electroporation; electroporation ... See more keywords
Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9740-4_11
Abstract: Transdermal delivery of gene medicine holds a great promise in gene therapy, and electroporation-mediated method is a high-efficiency drug transfer technique. Traditional skin electroporation approaches require high voltage that may cause severe injure. In this… read more here.
Keywords: transdermal delivery; nucleic acid; delivery; electroporation ... See more keywords
Isolation and Nanoscale Electroporation of Primary Neuronal Cultures In Situ.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9740-4_15
Abstract: Developing effective gene therapies for disorders of the central nervous system (CNS) is extremely challenging due to the lack of safe and efficient gene delivery methods to neurons and glial cells, hampering the study of… read more here.
Keywords: isolation nanoscale; neuronal cultures; nanoscale electroporation; electroporation ... See more keywords
Electroporation Transformation Protocol for Phaeodactylum tricornutum.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9740-4_17
Abstract: Genetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear transformation method for the model diatom Phaeodactylum tricornutum using an electroporation system, and… read more here.
Keywords: electroporation; electroporation transformation; phaeodactylum tricornutum; transformation ... See more keywords