Inclusion of MERS‐spike protein ELISA in algorithm to determine serologic evidence of MERS‐CoV infection
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Medical Virology"
DOI: 10.1002/jmv.24948
Abstract: The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS‐CoV) in subjects with potential infections with the virus has included screening by indirect… read more here.
Keywords: evidence; elisa; spike protein; inclusion mers ... See more keywords
Establishment of an indirect ELISA method for antibody detection of porcine pseudorabies by recombinant gB, gC, and gD proteins
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28228
Abstract: Pseudorabies virus (PRV), as a neuroherpes virus, leads to heavy economic losses in the pig industry worldwide. This study was designed to establish recombinant PRV glycoprotein B (gB), C, and D proteins as PRV diagnostic… read more here.
Keywords: establishment indirect; indirect elisa; recombinant proteins; elisa method ... See more keywords
Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9550-9_6
Abstract: The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is… read more here.
Keywords: elisa; elisa protocol; malaria surveillance; serological profiling ... See more keywords
Development of a New Monoclonal Antibody by More Active Enramycin A and Indirect Competitive ELISA for the Detection of Enramycin in Edible Animal Tissues
Sign Up to like & getrecommendations! Published in 2019 at "Food Analytical Methods"
DOI: 10.1007/s12161-019-01504-9
Abstract: In this research, we developed a new sensitive and specific monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect enramycin residues in edible animal tissues. Enramycin has two main components, Er-A and Er-B.… read more here.
Keywords: enramycin; detection; indirect competitive; elisa ... See more keywords
Preparation of a generic monoclonal antibody and development of a highly sensitive indirect competitive ELISA for the detection of phenothiazines in animal feed.
Sign Up to like & getrecommendations! Published in 2017 at "Food chemistry"
DOI: 10.1016/j.foodchem.2016.11.062
Abstract: In this study, a broadly specific monoclonal antibody was prepared and a sensitive monoclonal-based indirect competitive enzyme linked immunosorbent assay (ic-ELISA) was subsequently developed to determine the phenothiazines in animal feed with a simple sample… read more here.
Keywords: elisa; animal feed; phenothiazines animal; feed ... See more keywords
Development and characterization of a quantitative ELISA to detect anti-SARS-CoV-2 spike antibodies
Sign Up to like & getrecommendations! Published in 2021 at "Heliyon"
DOI: 10.1016/j.heliyon.2021.e08444
Abstract: A novel clinical assay for the detection and quantitation of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was adapted from an in-house, research-based enzyme-linked immunosorbent assay (ELISA). Development and validation were performed under… read more here.
Keywords: serology; elisa; covid; assay ... See more keywords
Development of an indirect enzyme-linked immunosorbent assay (ELISA) to differentiate antibodies against wild-type porcine reproductive and respiratory syndrome from the vaccine strain TJM-F92 based on a recombinant Nsp2 protein.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.09.001
Abstract: An accurate ELISA method to differentiate pigs infected with wild-type porcine reproductive and respiratory syndrome (PRRSV) strains from vaccinated ones would help to monitor PRRSV vaccination compliance. The recombinant protein GST-d120aa derived from the continuous… read more here.
Keywords: protein; tjm f92; elisa; type porcine ... See more keywords
Evaluation of ELISA and CLIA for Treponema pallidum specific antibody detection in China: A multicenter study.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2019.105742
Abstract: BACKGROUND Considering the rapid increase of syphilis infections in several countries including China, searching for a screening test with sufficient sensitivity and specificity is extremely urgent. The current study mainly researched the performance for Treponema… read more here.
Keywords: treponema pallidum; detection; study; elisa ... See more keywords
Reliability of techniques used in the diagnosis of canine visceral leishmaniasis by the national control program in Brazil: A survey in an area of recent transmission.
Sign Up to like & getrecommendations! Published in 2017 at "Preventive veterinary medicine"
DOI: 10.1016/j.prevetmed.2017.07.011
Abstract: One of the key components of the Brazilian Program for the Control of Visceral Leishmaniasis (PCLV) is the euthanasia of Leishmania-infected canine reservoirs, the detection of which depends on a screening procedure involving a Dual… read more here.
Keywords: field; elisa; dpp field; dpp ... See more keywords
Evaluation of three serological tests for diagnosis of bovine brucellosis in smallholder farms in Pakistan by estimating sensitivity and specificity using Bayesian latent class analysis.
Sign Up to like & getrecommendations! Published in 2018 at "Preventive veterinary medicine"
DOI: 10.1016/j.prevetmed.2017.11.002
Abstract: The present study aimed to estimate the herd-level sensitivity (Se) and specificity (Sp) of three commonly used serological tests in naturally-infected cattle and buffalo in smallholder farms in Pakistan. Between February and June 2015, a… read more here.
Keywords: farms pakistan; elisa; bayesian latent; serological tests ... See more keywords
Evaluation of ‘recombinant secretary antigens’ based ‘cocktail ELISA’ for the diagnosis of Johne's disease and to differentiate non-infected, infected and vaccinated goats in combination with indigenous ELISA test
Sign Up to like & getrecommendations! Published in 2018 at "Small Ruminant Research"
DOI: 10.1016/j.smallrumres.2018.06.005
Abstract: Abstract Johne's disease (JD) is the most widely distributed disease of the domestic livestock species world-wide. In the absence of control programs, bio-load of Mycobacterium avium subspecies paratuberculosis (MAP) had shown increasing trends in the… read more here.
Keywords: infected infected; johne disease; disease; elisa ... See more keywords