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   Articles with "elispot" as a keyword



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ELISPOT assays with pp65 peptides or whole HCMV antigen are reliable predictors of immune control of HCMV infection in seropositive kidney transplant recipients

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recommendations! Published in 2023 at "Journal of Medical Virology"

DOI: 10.1002/jmv.28507

Abstract: Human cytomegalovirus (HCMV) infection represents a major complication for solid organ transplant recipients. The aim of this study was to verify if the measurement of HCMV‐specific T‐cells could help to identify patients protected against HCMV… read more here.

Keywords: cfc idc; hcmv; hcmv infection; transplant recipients ... See more keywords
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CTL ELISPOT Assay and T Cell Detection.

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recommendations! Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1507-2_5

Abstract: Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T-cells that secrete cytokines and effector molecules, such as… read more here.

Keywords: elispot; elispot assay; antigen specific; ctl ... See more keywords
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Donor-specific elispot assay for predicting acute rejection and allograft function after kidney transplantation: a systematic review and meta-analysis.

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recommendations! Published in 2021 at "Clinical biochemistry"

DOI: 10.1016/j.clinbiochem.2021.04.011

Abstract: Acute rejection remains an important problem after kidney transplantation. Enzyme-linked immunosorbent spot (ELISPOT) assay has been investigated extensively and has shown promising results as a predictor of allograft rejection. The objective of this study was… read more here.

Keywords: elispot; acute rejection; rejection; kidney transplantation ... See more keywords
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Preformed donor‐reactive T cells that persist after ABO desensitization predict severe T cell‐mediated rejection after living donor kidney transplantation – a retrospective study

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recommendations! Published in 2019 at "Transplant International"

DOI: 10.1111/tri.13551

Abstract: Preformed donor‐reactive T cells are relatively resistant to standard immunosuppression and account for an increased incidence of T cell‐mediated rejection (TCMR) and inferior kidney allograft outcomes. We analyzed 150 living donor kidney transplant recipients (KTRs)… read more here.

Keywords: donor reactive; elispot; aboi ktrs; abo desensitization ... See more keywords
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Utility of Multi-target Nested PCR and ELISPOT Assays for the Detection of Paucibacillary Leprosy: A Possible Conclusion of Clinical Laboratory Misdiagnosis

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recommendations! Published in 2022 at "Frontiers in Cellular and Infection Microbiology"

DOI: 10.3389/fcimb.2022.814413

Abstract: The diagnosis of paucibacillary (PB) leprosy often possesses a diagnostic challenge, especially for pure neuritic and lesser skin lesions with the zero bacillary load, requiring a sensitive and accurate diagnostic tool. We have included 300… read more here.

Keywords: leprosy cases; pcr elispot; paucibacillary leprosy; clinical laboratory ... See more keywords
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Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium

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recommendations! Published in 2022 at "Frontiers in Immunology"

DOI: 10.3389/fimmu.2022.984642

Abstract: Influenza continues to be the most important cause of viral respiratory disease, despite the availability of vaccines. Today’s evaluation of influenza vaccines mainly focuses on the quantitative and functional analyses of antibodies to the surface… read more here.

Keywords: influenza specific; influenza; ifn; harmonization qualification ... See more keywords
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Acute and long-term immune responses to SARS-CoV-2 infection in unvaccinated children and young adults with inborn errors of immunity

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recommendations! Published in 2023 at "Frontiers in Immunology"

DOI: 10.3389/fimmu.2023.1084630

Abstract: Purpose To describe SARS-CoV-2 infection outcome in unvaccinated children and young adults with inborn errors of immunity (IEI) and to compare their specific acute and long-term immune responses with a sex-, age-, and severity-matched healthy… read more here.

Keywords: long term; infection; iei; sars cov ... See more keywords