ELISPOT assays with pp65 peptides or whole HCMV antigen are reliable predictors of immune control of HCMV infection in seropositive kidney transplant recipients
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28507
Abstract: Human cytomegalovirus (HCMV) infection represents a major complication for solid organ transplant recipients. The aim of this study was to verify if the measurement of HCMV‐specific T‐cells could help to identify patients protected against HCMV… read more here.
Keywords: cfc idc; hcmv; hcmv infection; transplant recipients ... See more keywords
CTL ELISPOT Assay and T Cell Detection.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1507-2_5
Abstract: Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T-cells that secrete cytokines and effector molecules, such as… read more here.
Keywords: elispot; elispot assay; antigen specific; ctl ... See more keywords
Donor-specific elispot assay for predicting acute rejection and allograft function after kidney transplantation: a systematic review and meta-analysis.
Sign Up to like & getrecommendations! Published in 2021 at "Clinical biochemistry"
DOI: 10.1016/j.clinbiochem.2021.04.011
Abstract: Acute rejection remains an important problem after kidney transplantation. Enzyme-linked immunosorbent spot (ELISPOT) assay has been investigated extensively and has shown promising results as a predictor of allograft rejection. The objective of this study was… read more here.
Keywords: elispot; acute rejection; rejection; kidney transplantation ... See more keywords
Development of an ELISpot Assay for the Assessment of AAV Peptides to Examine Immune Safety.
Sign Up to like & getrecommendations! Published in 2024 at "Human gene therapy"
DOI: 10.1089/hum.2023.180
Abstract: Adeno-associated virus (AAV)-based gene therapies have shown promise as novel treatments for rare genetic disorders such as Haemophilia A and Spinal Muscular Atrophy. However, cellular immune responses mediated by cytotoxic (CD8+) and helper (CD4+) T… read more here.
Keywords: aav; assay assessment; elispot; development elispot ... See more keywords
Preformed donor‐reactive T cells that persist after ABO desensitization predict severe T cell‐mediated rejection after living donor kidney transplantation – a retrospective study
Sign Up to like & getrecommendations! Published in 2019 at "Transplant International"
DOI: 10.1111/tri.13551
Abstract: Preformed donor‐reactive T cells are relatively resistant to standard immunosuppression and account for an increased incidence of T cell‐mediated rejection (TCMR) and inferior kidney allograft outcomes. We analyzed 150 living donor kidney transplant recipients (KTRs)… read more here.
Keywords: donor reactive; elispot; aboi ktrs; abo desensitization ... See more keywords
Utility of Multi-target Nested PCR and ELISPOT Assays for the Detection of Paucibacillary Leprosy: A Possible Conclusion of Clinical Laboratory Misdiagnosis
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2022.814413
Abstract: The diagnosis of paucibacillary (PB) leprosy often possesses a diagnostic challenge, especially for pure neuritic and lesser skin lesions with the zero bacillary load, requiring a sensitive and accurate diagnostic tool. We have included 300… read more here.
Keywords: leprosy cases; pcr elispot; paucibacillary leprosy; clinical laboratory ... See more keywords
Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.984642
Abstract: Influenza continues to be the most important cause of viral respiratory disease, despite the availability of vaccines. Today’s evaluation of influenza vaccines mainly focuses on the quantitative and functional analyses of antibodies to the surface… read more here.
Keywords: influenza specific; influenza; ifn; harmonization qualification ... See more keywords
Acute and long-term immune responses to SARS-CoV-2 infection in unvaccinated children and young adults with inborn errors of immunity
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2023.1084630
Abstract: Purpose To describe SARS-CoV-2 infection outcome in unvaccinated children and young adults with inborn errors of immunity (IEI) and to compare their specific acute and long-term immune responses with a sex-, age-, and severity-matched healthy… read more here.
Keywords: long term; infection; iei; sars cov ... See more keywords
Measurement of the Memory B Cell Response via Antibodies from Activated Cells
Sign Up to like & getrecommendations! Published in 2024 at "Antibodies"
DOI: 10.3390/antib13040081
Abstract: Background/Objectives: The body’s immune response to infections and vaccination leads to the formation of memory B cells (MBCs), which protect against future infections. MBCs circulate in the blood, and the strength of the MBC response… read more here.
Keywords: memory; antibody; response; mbc ... See more keywords