CTL ELISPOT Assay and T Cell Detection.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1507-2_5
Abstract: Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T-cells that secrete cytokines and effector molecules, such as… read more here.
Keywords: elispot; elispot assay; antigen specific; ctl ... See more keywords
B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.
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DOI: 10.1007/978-1-4939-8567-8_11
Abstract: Peripheral blood is commonly used to assess the cellular and humoral immune responses in clinical studies. It is a convenient sample to collect for immunological research as compared to the surgically excised and biopsied lymphoid… read more here.
Keywords: blood mononuclear; human peripheral; mononuclear cells; elispot assay ... See more keywords
Recommendations on ELISpot assay validation by the GCC.
Sign Up to like & getrecommendations! Published in 2022 at "Bioanalysis"
DOI: 10.4155/bio-2022-0010
Abstract: Gene therapy, cell therapy and vaccine research have led to an increased need to perform cellular immunity testing in a regulated environment to ensure the safety and efficacy of these treatments. The most common method… read more here.
Keywords: elispot assay; enzyme linked; assay validation; recommendations elispot ... See more keywords