Targeted Gene Knockdown in Early Embryos Using siRNA.
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DOI: 10.1007/978-1-4939-6988-3_14
Abstract: RNA interference is a convenient and highly effective technique to investigate the biological function of genes. Adequately designed RNA molecules introduced into an oocyte are able to bind specific endogenous mRNAs and trigger their degradation.… read more here.
Keywords: embryos using; using sirna; early embryos; gene ... See more keywords
Automated manipulation of zebrafish embryos using an electrothermal microgripper
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DOI: 10.1007/s00542-019-04728-1
Abstract: We present the first report on automated micro-manipulation of zebrafish embryos using an electrothermally-actuated microgripper. A five-bar linkage compliant microgripper, driven by a V-shaped electrothermal micro-actuator, is designed based on topological optimization. The electrothermally-actuated microgripper… read more here.
Keywords: embryos using; microgripper; zebrafish embryos; embryo ... See more keywords
Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
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DOI: 10.1016/j.stemcr.2019.01.019
Abstract: Summary Cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to… read more here.
Keywords: embryos using; apoptotic regulation; anti apoptotic; using cryopreserved ... See more keywords