Far Red‐Shifted CdTe Quantum Dots for Multicolour Stimulated Emission Depletion Nanoscopy
Sign Up to like & getrecommendations! Published in 2022 at "Chemphyschem"
DOI: 10.1002/cphc.202200698
Abstract: Abstract Stimulated emission depletion (STED) nanoscopy is a widely used nanoscopy technique. Two‐colour STED imaging in fixed and living cells is standardised today utilising both fluorescent dyes and fluorescent proteins. Solutions to image additional colours… read more here.
Keywords: red shifted; emission depletion; emission; stimulated emission ... See more keywords
Low‐power compact continuous‐wave stimulated emission depletion microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Biophotonics"
DOI: 10.1002/jbio.202200233
Abstract: Stimulated emission depletion (STED) microscopy can break the optical diffraction barrier and provide subdiffraction resolution. According to the STED superresolution imaging principle, the resolution of STED is positively related to the power of the depletion… read more here.
Keywords: low power; emission depletion; power; microscopy ... See more keywords
Lanthanide-Doped Nanoparticles for Stimulated Emission Depletion Nanoscopy
Sign Up to like & getrecommendations! Published in 2019 at "ACS Applied Nano Materials"
DOI: 10.1021/acsanm.9b01272
Abstract: Lanthanide-based photoluminescent emitters have gained strong attention over the past years because they provide certain advantages over organic fluorophores. Especially their excellent photostability has given rise to efforts in applying lanthanides for stimulated emission depletion… read more here.
Keywords: emission depletion; lanthanide doped; stimulated emission;
Stimulated Emission Depletion Spectroscopy of Color Centers in Hexagonal Boron Nitride
Sign Up to like & getrecommendations! Published in 2021 at "ACS Photonics"
DOI: 10.1021/acsphotonics.0c01917
Abstract: We demonstrate the use of Stimulated Emission Depletion (STED) spectroscopy to map the electron-optical-phonon sideband of the ground state of the radiative transition of color centers in hexagonal boron nitride emitting at 2.0–2.2 eV, with… read more here.
Keywords: hexagonal boron; spectroscopy; centers hexagonal; color centers ... See more keywords
Stimulated Emission Depletion Microscopy with Diamond Silicon Vacancy Centers
Sign Up to like & getrecommendations! Published in 2019 at "ACS Photonics"
DOI: 10.1021/acsphotonics.9b01135
Abstract: The spatial resolution and fluorescence signal amplitude in stimulated emission depletion (STED) microscopy is limited by the photostability of available fluorophores. Here, we show that negatively charged silicon vacancy (SiV) centers in diamond are promising… read more here.
Keywords: microscopy; vacancy; diamond; emission depletion ... See more keywords
Continuous-wave near-infrared stimulated-emission depletion microscopy using downshifting lanthanide nanoparticles
Sign Up to like & getrecommendations! Published in 2021 at "Nature Nanotechnology"
DOI: 10.1038/s41565-021-00927-y
Abstract: Stimulated-emission depletion (STED) microscopy has profoundly extended our horizons to the subcellular level1–3. However, it remains challenging to perform hours-long, autofluorescence-free super-resolution imaging in near-infrared (NIR) optical windows under facile continuous-wave laser depletion at low… read more here.
Keywords: microscopy; near infrared; depletion; emission depletion ... See more keywords
Recent research on stimulated emission depletion microscopy for reducing photobleaching
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Microscopy"
DOI: 10.1111/jmi.12698
Abstract: Stimulated emission depletion (STED) microscopy is a useful tool in investigation for super‐resolution realm. By silencing the peripheral fluorophores of the excited spot, leaving only the very centre zone vigorous for fluorescence, the effective point… read more here.
Keywords: research; stimulated emission; emission depletion; microscopy ... See more keywords
Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy
Sign Up to like & getrecommendations! Published in 2023 at "Neurophotonics"
DOI: 10.1117/1.nph.10.4.044402
Abstract: Abstract. Significance Stimulated emission depletion (STED) microscopy has been used to address a wide range of neurobiological questions in optically well-accessible samples, such as cell culture or brain slices. However, the application of STED to… read more here.
Keywords: dendritic spines; emission depletion; spines hippocampus; microscopy ... See more keywords
Coaxial illumination module of the stimulated-emission-depletion nanoscope.
Sign Up to like & getrecommendations! Published in 2022 at "Optics express"
DOI: 10.1364/oe.454350
Abstract: Stimulated-emission-depletion (STED) nanoscope achieves super-resolution imaging by using a donut-shaped depletion beam to darken the fluorophores around the excitation spot. As an important factor determining the resolution of imaging, the coaxiality between the excitation and… read more here.
Keywords: stimulated emission; illumination module; depletion; emission depletion ... See more keywords
An Efficient Aequorea victoria Green Fluorescent Protein for Stimulated Emission Depletion Super-Resolution Microscopy
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23052482
Abstract: In spite of their value as genetically encodable reporters for imaging in living systems, fluorescent proteins have been used sporadically for stimulated emission depletion (STED) super-resolution imaging, owing to their moderate photophysical resistance, which does… read more here.
Keywords: emission depletion; microscopy; emission; super resolution ... See more keywords