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Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0342-0_3
Abstract: Effective mutagenesis is critical for connecting traits of interest to specific plant genes. The development of site-directed mutagenesis and sequenced-indexed genetics resources in maize allows for targeted analysis of individual genes. These reverse genetics approaches…
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Keywords:
maize pollen;
genetics;
ems mutagenesis;
mutagenesis maize ... See more keywords
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Published in 2024 at "PLOS Genetics"
DOI: 10.1371/journal.pgen.1011377
Abstract: Chemical mutagenesis-driven forward genetic screens are pivotal in unveiling gene functions, yet identifying causal mutations behind phenotypes remains laborious, hindering their high-throughput application. Here, we reveal a non-uniform mutation rate caused by Ethyl Methane Sulfonate…
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Keywords:
mutagenesis probability;
machine learning;
learning enhanced;
genetic screens ... See more keywords
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2
Published in 2023 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1052569
Abstract: Ethyl methanesulfonate (EMS)-induced mutagenesis is a powerful tool to generate genetic resource for identifying untapped genes and characterizing the function of genes to understand the molecular basis of important agronomic traits. This review focuses on…
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Keywords:
ems mutagenesis;
abiotic stress;
stress tolerance;
plant ... See more keywords