Lighting up the brain: genetically encoded fluorescent sensors for imaging neurotransmitters and neuromodulators
Sign Up to like & getrecommendations! Published in 2018 at "Current Opinion in Neurobiology"
DOI: 10.1016/j.conb.2018.03.010
Abstract: Measuring the precise dynamics of specific neurotransmitters and neuromodulators in the brain is essential for understanding how information is transmitted and processed. Thanks to the development and optimization of various genetically encoded sensors, we are… read more here.
Keywords: lighting brain; genetically encoded; encoded fluorescent; neurotransmitters neuromodulators ... See more keywords
A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine
Sign Up to like & getrecommendations! Published in 2019 at "Neuron"
DOI: 10.1016/j.neuron.2019.02.037
Abstract: Norepinephrine (NE) is a key biogenic monoamine neurotransmitter involved in a wide range of physiological processes. However, its precise dynamics and regulation remain poorly characterized, in part due to limitations of available techniques for measuring NE in vivo.… read more here.
Keywords: vivo; grabne sensors; fluorescent sensor; encoded fluorescent ... See more keywords
Genetically Encoded Fluorescent Probe for Detecting Sirtuins in Living Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b05725
Abstract: Sirtuins are NAD+ dependent protein deacetylases, which are involved in many biological processes. We now report a novel genetically encoded fluorescent probe (EGFP-K85AcK) that responds to sirtuins in living cells. The probe design exploits a… read more here.
Keywords: fluorescent probe; probe; genetically encoded; encoded fluorescent ... See more keywords
SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical communications"
DOI: 10.1039/c7cc08740c
Abstract: We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria… read more here.
Keywords: sypher3s genetically; enhanced brightness; probe; genetically encoded ... See more keywords
Genetically encoded fluorescent tags
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e16-07-0504
Abstract: Genetically encoded fluorescent tags are protein sequences that can be fused to a protein of interest to render it fluorescent. These tags have revolutionized cell biology by allowing nearly any protein to be imaged by… read more here.
Keywords: fluorescent tags; genetically encoded; encoded fluorescent; biology ... See more keywords
Genetically encoded fluorescent sensors adapted to acidic pH highlight subdomains within the plant cell apoplast.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of experimental botany"
DOI: 10.1093/jxb/erac210
Abstract: Monitoring pH is one of the challenges in understanding diverse physiological regulations as well as ionic balance, especially in highly acidic environments such as the apoplast and the vacuole. To circumvent the poor efficiency of… read more here.
Keywords: cell; adapted acidic; encoded fluorescent; genetically encoded ... See more keywords
Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Neurochemistry"
DOI: 10.1111/jnc.15608
Abstract: The brain relies on many forms of dynamic activities in individual neurons, from synaptic transmission to electrical activity and intracellular signaling events. Monitoring these neuronal activities with high spatiotemporal resolution in the context of animal… read more here.
Keywords: imaging neuronal; neuronal dynamics; encoded fluorescent; genetically encoded ... See more keywords
In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abq4915
Abstract: Nitrate (NO3−) uptake and distribution are critical to plant life. Although the upstream regulation of NO3− uptake and downstream responses to NO3− in a variety of cells have been well studied, it is still not… read more here.
Keywords: encoded fluorescent; genetically encoded; fluorescent biosensor; distribution ... See more keywords