A comprehensive evaluation of single-end sequencing data analyses for environmental microbiome research.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of microbiology"
DOI: 10.1007/s00203-021-02597-9
Abstract: Illumina sequencing platforms have been widely used for amplicon-based environmental microbiome research. Analyses of amplicon data of environmental samples, generated from Illumina MiSeq platform illustrate the reverse (R2) reads in the PE datasets to have… read more here.
Keywords: sequencing data; microbiome research; environmental microbiome; single end ... See more keywords
Long fragments achieve lower base quality in Illumina paired-end sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-39076-7
Abstract: Illumina’s technology provides high quality reads of DNA fragments with error rates below 1/1000 per base. Sequencing runs typically generate millions of reads in which the vast majority of the reads has an average error… read more here.
Keywords: quality; paired end; end sequencing; base ... See more keywords
Can Targeting Non-Contiguous V-Regions With Paired-End Sequencing Improve 16S rRNA-Based Taxonomic Resolution of Microbiomes?: An In Silico Evaluation
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2019.00653
Abstract: Background: Next-generation sequencing (NGS) technologies have enabled probing of microbial diversity in different environmental niches with unprecedented sequencing depth. However, due to read-length limitations of popular NGS technologies, 16S amplicon sequencing-based microbiome studies rely on… read more here.
Keywords: paired end; end sequencing; 16s rrna; taxonomic resolution ... See more keywords