Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c05353
Abstract: Untargeted label-free interrogation of proteins in their functional form directly from their physiological environment promises to transform life sciences research by providing unprecedented insight into their transient interactions with other biomolecules and xenobiotics. Native ambient… read more here.
Keywords: mass spectrometry; endogenous protein; protein assemblies;
Improved split fluorescent proteins for endogenous protein labeling
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-00494-8
Abstract: Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell–cell contact. To expand this toolset, we have developed a screening strategy for the direct… read more here.
Keywords: split fluorescent; protein labeling; fluorescent proteins; endogenous protein ... See more keywords
EndoBind detects endogenous protein-protein interactions in real time
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-02600-5
Abstract: We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples… read more here.
Keywords: interactions real; real time; protein interactions; protein protein ... See more keywords
Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach
Sign Up to like & getrecommendations! Published in 2021 at "eLife"
DOI: 10.7554/elife.75050
Abstract: The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins by homology-directed repair in a variety of species. Despite its revolutionary success, there remains an urgent need for increased simplicity and efficiency of… read more here.
Keywords: cas9; endogenous protein; protein tagging; crispr cas9 ... See more keywords