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   Articles with "endogenous proteins" as a keyword



Endogenous Proteins Modulation in Live Cells with Small Molecules and Light

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recommendations! Published in 2022 at "ChemBioChem"

DOI: 10.1002/cbic.202200244

Abstract: Protein modulation by light illumination enables the investigation of biological function in high spatiotemporal precision. Compared to genetic methods, the small molecule approach is uniquely suited for modulating endogenous proteins. Endogenous protein modulation in live… read more here.

Keywords: molecules light; endogenous proteins; live cells; small molecules ... See more keywords
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Advances in targeted degradation of endogenous proteins

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recommendations! Published in 2019 at "Cellular and Molecular Life Sciences"

DOI: 10.1007/s00018-019-03112-6

Abstract: Protein silencing is often employed as a means to aid investigations in protein function and is increasingly desired as a therapeutic approach. Several types of protein silencing methodologies have been developed, including targeting the encoding… read more here.

Keywords: protein; degradation endogenous; degradation; advances targeted ... See more keywords
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Spatially Resolved Mapping of Endogenous Proteins and RNA in Living Cells

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recommendations! Published in 2017 at "Biophysical Journal"

DOI: 10.1016/j.bpj.2016.11.063

Abstract: I will describe our efforts to build molecular tools for studying mitochondria and the synapse in living cells and tissue. These might include tools for proteomic mapping, transcriptomic mapping, and/or neural circuit mapping. read more here.

Keywords: resolved mapping; endogenous proteins; proteins rna; living cells ... See more keywords

A Normalization Method Utilizing Endogenous Proteins for Quantitative Proteomics.

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recommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"

DOI: 10.1021/jasms.0c00012

Abstract: We developed a normalization method utilizing the expression levels of a panel of endogenous proteins as normalization standards (EPNS herein). We tested the validity of the method using two sets of TMT-labeled data and found… read more here.

Keywords: normalization method; method; normalization; method utilizing ... See more keywords
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Cell biology: Depleting endogenous proteins

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recommendations! Published in 2018 at "Nature Methods"

DOI: 10.1038/nmeth.4573

Abstract: Using antibodies to target specific proteins for rapid degradation is a versatile approach for studying the function of endogenous proteins. read more here.

Keywords: cell biology; biology; depleting endogenous; biology depleting ... See more keywords

NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins

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recommendations! Published in 2021 at "Nature Communications"

DOI: 10.1038/s41467-021-20963-5

Abstract: The Neisseria meningitidis protein FrpC contains a self-processing module (SPM) undergoing autoproteolysis via an aspartic anhydride. Herein, we establish NeissLock, using a binding protein genetically fused to SPM. Upon calcium triggering of SPM, the anhydride… read more here.

Keywords: protein; covalent; neisslock provides; covalent targeting ... See more keywords

Intracellular protein editing enables incorporation of noncanonical residues in endogenous proteins.

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recommendations! Published in 2025 at "Science"

DOI: 10.1126/science.adr5499

Abstract: The ability to study proteins in their native cellular context is crucial to our understanding of biology. In this work, we report a technology for intracellular protein editing, drawing from split intein-mediated protein splicing, genetic… read more here.

Keywords: intracellular protein; protein editing; protein; editing enables ... See more keywords

Identifying Endogenous Proteins of Perennial Ryegrass (Lolium perenne) with Ex Vivo Antioxidant Activity

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recommendations! Published in 2025 at "Proteomes"

DOI: 10.3390/proteomes13010008

Abstract: Background: During the initial steps of green biorefining aimed at protein recovery, endogenous proteins and enzymes, along with, e.g., phytochemical constituents, are decompartmentalized into a green juice. This creates a highly dynamic environment prone to… read more here.

Keywords: vivo; perenne; perennial ryegrass; antioxidant activity ... See more keywords

Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

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recommendations! Published in 2022 at "eLife"

DOI: 10.7554/elife.77987

Abstract: Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific… read more here.

Keywords: auxin inducible; inducible degrons; proteins mouse; endogenous proteins ... See more keywords