Endogenous Proteins Modulation in Live Cells with Small Molecules and Light
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DOI: 10.1002/cbic.202200244
Abstract: Protein modulation by light illumination enables the investigation of biological function in high spatiotemporal precision. Compared to genetic methods, the small molecule approach is uniquely suited for modulating endogenous proteins. Endogenous protein modulation in live… read more here.
Keywords: molecules light; endogenous proteins; live cells; small molecules ... See more keywords
Advances in targeted degradation of endogenous proteins
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DOI: 10.1007/s00018-019-03112-6
Abstract: Protein silencing is often employed as a means to aid investigations in protein function and is increasingly desired as a therapeutic approach. Several types of protein silencing methodologies have been developed, including targeting the encoding… read more here.
Keywords: protein; degradation endogenous; degradation; advances targeted ... See more keywords
Spatially Resolved Mapping of Endogenous Proteins and RNA in Living Cells
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DOI: 10.1016/j.bpj.2016.11.063
Abstract: I will describe our efforts to build molecular tools for studying mitochondria and the synapse in living cells and tissue. These might include tools for proteomic mapping, transcriptomic mapping, and/or neural circuit mapping. read more here.
Keywords: resolved mapping; endogenous proteins; proteins rna; living cells ... See more keywords
A Normalization Method Utilizing Endogenous Proteins for Quantitative Proteomics.
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DOI: 10.1021/jasms.0c00012
Abstract: We developed a normalization method utilizing the expression levels of a panel of endogenous proteins as normalization standards (EPNS herein). We tested the validity of the method using two sets of TMT-labeled data and found… read more here.
Keywords: normalization method; method; normalization; method utilizing ... See more keywords
Cell biology: Depleting endogenous proteins
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DOI: 10.1038/nmeth.4573
Abstract: Using antibodies to target specific proteins for rapid degradation is a versatile approach for studying the function of endogenous proteins. read more here.
Keywords: cell biology; biology; depleting endogenous; biology depleting ... See more keywords
NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins
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DOI: 10.1038/s41467-021-20963-5
Abstract: The Neisseria meningitidis protein FrpC contains a self-processing module (SPM) undergoing autoproteolysis via an aspartic anhydride. Herein, we establish NeissLock, using a binding protein genetically fused to SPM. Upon calcium triggering of SPM, the anhydride… read more here.
Keywords: protein; covalent; neisslock provides; covalent targeting ... See more keywords
Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons
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DOI: 10.7554/elife.77987
Abstract: Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific… read more here.
Keywords: auxin inducible; inducible degrons; proteins mouse; endogenous proteins ... See more keywords