Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202311240
Abstract: Nanoparticle‐mediated intracellular delivery of oligonucleotides is a complex phenomenon that depends on the architecture and the intracellular trafficking of the engineered nanoparticles. Unravelling the molecular arrangements of oligonucleotides within the nanoparticles as well as their… read more here.
Keywords: live cells; escape nanoparticles; forming oligonucleotide; escape ... See more keywords
Beyond the Endosomal Bottleneck: Understanding the Efficiency of mRNA/LNP Delivery
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202404510
Abstract: The development of lipid nanoparticles (LNP) holds great promise for vaccine and therapeutic applications. However, ineffective transport of nucleic acid cargo from endosomes to the cytosol, termed “endosomal escape,” has remained a bottleneck to achieving… read more here.
Keywords: mrna lnp; efficiency; delivery; lnp ... See more keywords
Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200167
Abstract: Crossing the cellular membrane and delivering active pharmaceuticals or biologicals into the cytosol of cells is an essential step in the development of nanomedicines. One of the most important intracellular processes regarding the cellular uptake… read more here.
Keywords: tracking endosomal; endosomal escape; closer look; escape closer ... See more keywords
Effective mRNA Protection by Poly(L-Ornithine) Synergizes with Endosomal Escape Functionality of a Charge-Conversion Polymer Toward Maximizing mRNA Introduction Efficiency.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular rapid communications"
DOI: 10.1002/marc.202100754
Abstract: For efficient delivery of messenger (m)RNA, delivery carriers need two major functions: protecting mRNA from nucleases and translocating mRNA from endolysosomes to the cytoplasm. Herein, these two complementary functionalities were integrated into a single polyplex… read more here.
Keywords: mrna polyplex; endosomal escape; charge conversion; poly ornithine ... See more keywords
Imidazolyl Lipids Enhanced LNP Endosomal Escape for Ferroptosis RNAi Treatment of Cancer.
Sign Up to like & getrecommendations! Published in 2024 at "Small"
DOI: 10.1002/smll.202402362
Abstract: Treatments for cancer that incorporate small interfering RNA (siRNA) to target iron-dependent ferroptosis are thought to be highly promising. However, creating a reliable and clinically feasible siRNA delivery system continues to be a major obstacle… read more here.
Keywords: cancer; treatment; ferroptosis; sirna ... See more keywords
Reconstructed chitosan with alkylamine for enhanced gene delivery by promoting endosomal escape.
Sign Up to like & getrecommendations! Published in 2020 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2019.115339
Abstract: Poor buffering capacity of chitosan (CS) results in insufficient intracellular gene release which poses the major barrier in gene delivery. Herein, we reconstructed pristine CS with propylamine (PA), (diethylamino) propylamine (DEAPA), and N, N-dimethyl- dipropylenetriamine… read more here.
Keywords: alkylamine; delivery; gene delivery; gene ... See more keywords
Endosomal escape of peptide-photosensitizer conjugates is affected by amino acid sequences near the photosensitizer.
Sign Up to like & getrecommendations! Published in 2020 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.0c00046
Abstract: Cell-penetrating peptides (CPPs) are widely used for the intracellular delivery of peptides and proteins, but CPP fusion peptides and proteins are often transported by endocytosis and trapped in endosomes. Photochemical internalization (PCI) is a method… read more here.
Keywords: photosensitizer; amino acid; photosensitizer conjugates; near photosensitizer ... See more keywords
Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP.
Sign Up to like & getrecommendations! Published in 2021 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.0c01767
Abstract: The ability for biologics to access intracellular targets hinges on the translocation of active, unmodified proteins. This is often achieved using nanoscale formulations, which enter cells through endocytosis. This uptake mechanism often limits the therapeutic… read more here.
Keywords: caspase; escape; delivery; polymer ... See more keywords
Dynamic Continuum of Nanoscale Peptide Assemblies Facilitates Endocytosis and Endosomal Escape.
Sign Up to like & getrecommendations! Published in 2021 at "Nano letters"
DOI: 10.1021/acs.nanolett.1c01029
Abstract: Alkaline phosphatase (ALP) enables intracellular targeting by peptide assemblies, but how the ALP substrates enter cells remains elusive. Here we show that nanoscale phosphopeptide assemblies cluster ALP to enable caveolae-mediated endocytosis (CME) and endosomal escape.… read more here.
Keywords: peptide assemblies; continuum nanoscale; escape; endocytosis ... See more keywords
Cytosolic delivery of macromolecules in live human cells using the combined endosomal escape activities of a small molecule and cell penetrating peptides.
Sign Up to like & getrecommendations! Published in 2019 at "ACS chemical biology"
DOI: 10.1021/acschembio.9b00585
Abstract: Ineffective cellular delivery is a common problem in numerous biological applications. Developing delivery reagents that work robustly in a variety of experimental settings remains a challenge. Herein, we report how peptides derived from the prototypical… read more here.
Keywords: penetrating peptides; small molecule; delivery; cell penetrating ... See more keywords
Engineering Lipid Nanoparticles for Enhanced Intracellular Delivery of mRNA through Inhalation.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c05647
Abstract: Despite lipid nanoparticles' (LNPs) success in the effective and safe delivery of mRNA vaccines, an inhalation-based mRNA therapy for lung diseases remains challenging. LNPs tend to disintegrate due to shear stress during aerosolization, leading to… read more here.
Keywords: lipid nanoparticles; endosomal escape; delivery mrna; delivery ... See more keywords