Genome Editing in Mouse and Rat by Electroporation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7128-2_7
Abstract: Many knock-out/knock-in mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Microinjection… read more here.
Keywords: engineered endonucleases; electroporation; genome editing; mouse rat ... See more keywords
Generation of genetically-engineered animals using engineered endonucleases
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Pharmacal Research"
DOI: 10.1007/s12272-018-1037-z
Abstract: The key to successful drug discovery and development is to find the most suitable animal model of human diseases for the preclinical studies. The recent emergence of engineered endonucleases is allowing for efficient and precise… read more here.
Keywords: genetically engineered; engineered endonucleases; generation genetically; human diseases ... See more keywords
Use of gene-editing technology to introduce targeted modifications in pigs
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Animal Science and Biotechnology"
DOI: 10.1186/s40104-017-0228-7
Abstract: Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful… read more here.
Keywords: gene editing; pig production; site specific; engineered endonucleases ... See more keywords