Engineering enzyme access tunnels.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2019.04.008
Abstract: Enzymes are efficient and specific catalysts for many essential reactions in biotechnological and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic efficiency, stability or specificity required for these industrial processes. The… read more here.
Keywords: engineering; tunnels channels; access tunnels; engineering enzyme ... See more keywords
Boosted activity by engineering the enzyme microenvironment in cascade reaction: A molecular understanding
Sign Up to like & getrecommendations! Published in 2021 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2021.06.004
Abstract: Engineering of enzyme microenvironment can surprisingly boost the apparent activity. However, the underlying regulation mechanism is not well-studied at a molecular level so far. Here, we present a modulation of two model enzymes of cytochrome… read more here.
Keywords: engineering enzyme; enzyme microenvironment; activity; cascade reaction ... See more keywords
Multilayer Engineering of Enzyme Cascade Catalysis for One-Pot Preparation of Nylon Monomers from Renewable Fatty Acids
Sign Up to like & getrecommendations! Published in 2020 at "ACS Catalysis"
DOI: 10.1021/acscatal.9b05426
Abstract: Enzyme cascade catalysis has critical problems in obtaining the high concentrations of products, such as the low stabilities and activities of biocatalysts and the inhibition by hydrophobic reactan... read more here.
Keywords: enzyme cascade; catalysis; engineering enzyme; cascade catalysis ... See more keywords
Engineering an Enzyme for Direct Electrical Monitoring of Activity.
Sign Up to like & getrecommendations! Published in 2019 at "ACS nano"
DOI: 10.1021/acsnano.9b06875
Abstract: Proteins have been shown to be electrically-conductive if tethered to an electrode by means of a specific binding agent, allowing single molecules to be wired into an electrical sensing circuit. Such circuits allow enzymes to… read more here.
Keywords: enzyme direct; engineering enzyme; monitoring activity; activity ... See more keywords
Engineering enzyme microenvironments for enhanced biocatalysis.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Society reviews"
DOI: 10.1039/c8cs00085a
Abstract: Protein engineering provides a means to alter protein structure leading to new functions. Much work has focused on the engineering of enzyme active sites to enhance catalytic activity, however there is an increasing trend towards… read more here.
Keywords: engineering; microenvironments enhanced; engineering enzyme; enzyme microenvironments ... See more keywords