Estimating kinetic constants in the Michaelis–Menten model from one enzymatic assay using Approximate Bayesian Computation
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DOI: 10.1002/1873-3468.13531
Abstract: The Michaelis–Menten equation is one of the most extensively used models in biochemistry for studying enzyme kinetics. However, this model requires at least a couple (e.g., eight or more) of measurements at different substrate concentrations… read more here.
Keywords: kinetic constants; michaelis menten; estimating kinetic; enzymatic assay ... See more keywords
A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases
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DOI: 10.1007/s10529-019-02760-9
Abstract: Objective The development of an enzymatic assay for the specific quantification of the C1-oxidation product, i.e. gluconic acid of cellulose active lytic polysaccharide monooxygenases (LPMOs). Results In combination with a β-glucosidase, the spectrophotometrical assay can… read more here.
Keywords: oxidation; enzymatic assay; assay; polysaccharide monooxygenases ... See more keywords
A Novel Control-Theory-Based Approach to Scheduling of High-Throughput Screening System for Enzymatic Assay
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DOI: 10.1109/tsmc.2022.3161643
Abstract: Nowadays, high-throughput screening (HTS) systems are widely used in pharmaceutical industries and laboratories for the discovery of new drugs and biomedical substances. It is important to efficiently schedule them so as to reduce the cost.… read more here.
Keywords: system; enzymatic assay; system enzymatic; throughput screening ... See more keywords