Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations
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DOI: 10.1186/s40643-017-0143-y
Abstract: BackgroundSortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (ranging from several milligrams… read more here.
Keywords: staphylococcus aureus; escherichia coli; enzymatic characterizations; expression ... See more keywords