Articles with "enzymatic method" as a keyword



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Enzymatic method and its validation for the micromolar assay of glucose in human serum samples.

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Published in 2019 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2019.113536

Abstract: This work depicts the novel chromogenic system for the assay of glucose in blood samples connected with the chelate formation of sulfosalicylic acid (SSA) with iron (Fe(III)) using glucose oxidase (GOD) presented. The purple-colored Fe-SSA… read more here.

Keywords: enzymatic method; method; serum; assay glucose ... See more keywords
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A new enzymatic method for extracting diatoms from organs of suspected drowning cases using papain: Optimal digestion and first practical application.

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Published in 2019 at "Forensic science international"

DOI: 10.1016/j.forsciint.2019.02.008

Abstract: Diatom analysis is very effective for positive diagnosis of water inhalation in drowning. However, conventional strong acid diatom testing is laborious and potentially dangerous. We propose a simple, fast, and safe protocol using inexpensive reagents… read more here.

Keywords: using papain; enzymatic method; digestion; method ... See more keywords
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Development and Validation of an Enzymatic Method To Determine Stevioside Content from Stevia rebaudiana.

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Published in 2017 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.6b05793

Abstract: An enzymatic method for specific determination of stevioside content was established. Recombinant β-glucosidase BT_3567 (rBT_3567) from Bacteroides thetaiotaomicron HB-13 exhibited selective hydrolysis of stevioside at β-1,2-glycosidic bond to yield rubusoside and glucose. Coupling of this… read more here.

Keywords: stevioside content; content stevia; enzymatic method; stevioside ... See more keywords
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A novel enzymatic method for synthesis of glycopeptides carrying natural eukaryotic N-glycans.

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Published in 2017 at "Chemical communications"

DOI: 10.1039/c7cc04362g

Abstract: A novel enzymatic approach was developed for facile production of glycopeptides carrying natural eukaryotic N-glycans. In this approach, peptides can be GlcNAcylated at one or two natural N-glycosylation sites via two-step enzymatic reactions catalyzed by… read more here.

Keywords: carrying natural; glycopeptides carrying; enzymatic method; eukaryotic glycans ... See more keywords
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Validation and kinetic of enzymatic method for the detection of organophosphate insecticides based on cholinesterase inhibition

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Published in 2019 at "Toxicology Mechanisms and Methods"

DOI: 10.1080/15376516.2019.1669248

Abstract: Abstract Kinetic and validation of the enzymatic method for the determination of fenitrothion organophosphorus based on cholinesterase inhibition were studied. A Linear relationship was obtained with a determination coefficient R2 of 0.9989 suggesting that the… read more here.

Keywords: cholinesterase inhibition; enzymatic method; detection; inhibition ... See more keywords
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Isolation and Quantification of the Hepatoprotective Flavonoids From Scleromitron diffusum (Willd.) R. J. Wang With Bio-Enzymatic Method Against NAFLD by UPLC–MS/MS

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Published in 2022 at "Frontiers in Pharmacology"

DOI: 10.3389/fphar.2022.890148

Abstract: Flavonoids were the major phytochemicals against hepatic peroxidative injury in Scleromitron diffusum (Willd.) R. J. Wang with an inventive bio-enzymatic method by our group (LU500041). Firstly, the total flavonoids from Scleromitron diffusum (Willd.) R. J.… read more here.

Keywords: diffusum willd; bio enzymatic; enzymatic method; willd wang ... See more keywords