CXCR4 expression by mesenchymal stromal cells is lost after use of enzymatic dissociation agents, but preserved by use of non-enzymatic methods
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Hematology"
DOI: 10.1007/s12185-020-03043-0
Abstract: In recent years, multipotent mesenchymal stromal cells (MSCs) have demonstrated tremendous potential for use in regenerative medicine. CXCR4, the receptor for CXCL12, is highly expressed by bone marrow (BM) MSCs and the CXCR4/CXCL12 axis has… read more here.
Keywords: dissociation; cxcr4 expression; enzymatic dissociation; enzymatic methods ... See more keywords
Chemical and Enzymatic Methods for Post-Translational Protein–Protein Conjugation
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c00129
Abstract: Fusion proteins play an essential role in the biosciences but suffer from several key limitations, including the requirement for N-to-C terminal ligation, incompatibility of constituent domains, incorrect folding, and loss of biological activity. This perspective… read more here.
Keywords: protein protein; post translational; enzymatic methods; chemical enzymatic ... See more keywords
Comparison of LC-MS/MS and Enzymatic Methods for the Determination of Total Choline and Total Carnitine in Infant Formula and Milk Products.
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DOI: 10.1093/jaoacint/qsaa060
Abstract: BACKGROUND Choline and l-carnitine are classified as pseudo-vitamins because of their conditionally essential status. As they are involved in multiple physiological metabolic pathways in the human body, they are routinely fortified in infant and adult… read more here.
Keywords: milk; choline; total carnitine; total choline ... See more keywords
Determination of pyrroloquinoline quinone by enzymatic and LC-MS/MS methods to clarify its levels in foods
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DOI: 10.1371/journal.pone.0209700
Abstract: Pyrroloquinoline quinone (PQQ) is believed to be a new B vitamin-like compound, and PQQ supplementation has received attention as a possible treatment for diseases including dementia and diabetes. However, the distribution of PQQ in foods… read more here.
Keywords: determination pyrroloquinoline; pyrroloquinoline quinone; pqq; enzymatic methods ... See more keywords
Enzymatic Methods for the Site-Specific Radiolabeling of Targeting Proteins
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DOI: 10.3390/molecules26123492
Abstract: Site-specific conjugation of proteins is currently required to produce homogenous derivatives for medicine applications. Proteins derivatized at specific positions of the polypeptide chain can actually show higher stability, superior pharmacokinetics, and activity in vivo, as… read more here.
Keywords: medicine; specific radiolabeling; site specific; enzymatic methods ... See more keywords