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Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12833
Abstract: Shewanella oneidensis, a Gram‐negative γ‐proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a…
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Keywords:
yellow enzyme;
shewanella oneidensis;
enzyme;
meisenheimer complex ... See more keywords
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Published in 2022 at "Advanced materials"
DOI: 10.1002/adma.202200871
Abstract: Natural enzyme-based catalytic cascades hold great promise for cancer therapy, but their clinical utility is greatly hindered by the loss of their functions during in vivo delivery. Herein, we report a plasmonic tri-enzyme integrated metal-organic…
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Keywords:
therapy;
tri enzyme;
high efficiency;
enzyme cascades ... See more keywords
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Published in 2022 at "Archiv Der Pharmazie"
DOI: 10.1002/ardp.202200121
Abstract: As an attempt to contribute to the efforts of combating the pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) responsible for COVID‐19, new analogs of the repurposed drug nitazoxanide which showed promising inhibitory efficacy…
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Keywords:
inhibition;
protease;
molecular modeling;
enzyme ... See more keywords
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Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1660
Abstract: Hunter syndrome (Mucopolysaccharidosis II, MPS II) is an X‐linked lysosomal storage disease produced by the deficiency of the lysosomal enzyme iduronate‐2‐sulfatase (IDS). Currently, MPS II patients are mainly treated with enzyme replacement therapy (ERT) using…
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Keywords:
production;
enzyme;
production characterization;
pichia pastoris ... See more keywords
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Published in 2023 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.2467
Abstract: Paraoxonase 1 (PON1) was purified 148.80-fold in 37.92% yield by hydrophobic interaction chromatography technique. The purity of PON1 was checked by SDS PAGE with a single band of 43 kDa. The in vitro effects of…
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Keywords:
molecular docking;
channel blockers;
calcium channel;
molecular dynamics ... See more keywords
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Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202300143
Abstract: We present a paper microfluidic device capable of conducting enzyme-linked assays: a microfluidic enzyme-linked paper analytical device (μEL-PAD). The system exploits a wash-free sandwich coupling to form beads/analyte/enzyme complexes, which are subsequently added to the…
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Keywords:
dna;
enzyme linked;
enzyme;
device ... See more keywords
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Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28014
Abstract: Biocatalysis in high‐concentration organic solvents (OSs) offers many advantages, but realizing this process remains a huge challenge. An R‐selective ω‐amine transaminase variant (AcATAM2) exhibited high activity toward 50 g/L pro‐sitagliptin ketone 1‐[1‐piperidinyl]‐4‐[2,4,5‐trifluorophenyl]‐1,3‐butanedione (PTfpB). However, AcATAM2 displayed…
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Keywords:
enzyme;
concentration organic;
biocatalysis high;
dmso ... See more keywords
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1
Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3282
Abstract: Electrospun nanofibers have gained much attention for enzyme immobilization due to their high surface‐to‐volume ratio. In this study, urease was immobilized on chitosan/poly(vinyl alcohol) (PVA) nanofibers by both adsorption and crosslinking methods. In order to…
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Keywords:
immobilization;
electrospun;
immobilization urease;
enzyme ... See more keywords
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Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201600703
Abstract: Cellular metabolism involves complex sequences of organized enzymatic reactions, known as metabolic pathways, that convert substrates into readily usable materials. In nature, these enzymatic complexes are organized in a well‐defined manner so that the cascade…
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Keywords:
enzyme cascades;
acid templated;
enzyme;
templated enzyme ... See more keywords
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Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800337
Abstract: Spatial organization of multiple enzymes at specific positions for a controlled reaction cascade has attracted wide attention in recent years. Here, we report the construction of a biomimetic enzyme cascade organized on DNA triangle prism…
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Keywords:
dna;
enzyme cascade;
enzyme;
generation ... See more keywords
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Published in 2020 at "ChemCatChem"
DOI: 10.1002/cctc.201902089
Abstract: NADP+‐dependent formate dehydrogenases (FDHs) are biotechnologically relevant enzymes for cofactors regeneration in industrial processes employing redox biocatalysts. Their effective applicability is however hampered by the low cofactor and substrate affinities of the few enzymes described…
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Keywords:
dependent formate;
nadp dependent;
formate dehydrogenase;
enzyme ... See more keywords