Determination of miRNA derived from exosomes of prostate cancer via toehold-aided cyclic amplification combined with HRP enzyme catalysis and magnetic nanoparticles.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114336
Abstract: MicroRNAs (miRNAs) play a significant role in tumorigenesis and tumor development. Exosomal microRNA-141 (miRNA-141, miR-141) has been reported to be overexpressed in prostate cancer (PCa) and has become a potential biomarker for the diagnosis of… read more here.
Keywords: hrp enzyme; toehold aided; prostate cancer; enzyme catalysis ... See more keywords
'Negative' and 'positive catalysis': complementary principles that shape the catalytic landscape of enzymes.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.09.013
Abstract: Our understanding of enzyme catalysis is dominated by transition state theory. According to this concept, an enzymatic reaction is guided along a desired reaction coordinate through the stabilization of favorable transition state. But how much… read more here.
Keywords: landscape enzymes; catalytic landscape; catalysis; enzyme catalysis ... See more keywords
Exploring mechanism of enzyme catalysis by on-chip transient kinetics coupled with global data analysis and molecular modeling
Sign Up to like & getrecommendations! Published in 2021 at "Chem"
DOI: 10.1016/j.chempr.2021.02.011
Abstract: Summary The ability to engineer enzymes for industrial and biomedical applications is primarily limited by a paucity of mechanistic understanding. To gain insight into the mechanisms of enzyme catalysis, one must screen enormous numbers of… read more here.
Keywords: transient; mechanism enzyme; transient kinetics; exploring mechanism ... See more keywords
Entropy and Enzyme Catalysis.
Sign Up to like & getrecommendations! Published in 2017 at "Accounts of chemical research"
DOI: 10.1021/acs.accounts.6b00321
Abstract: The role played by entropy for the enormous rate enhancement achieved by enzymes has been debated for many decades. There are, for example, several confirmed cases where the activation free energy is reduced by around… read more here.
Keywords: entropy; catalysis; enzyme catalysis; reaction ... See more keywords
Modulating Polarization of Perovskite-Based Heterostructures via In Situ Semiconductor Generation and Enzyme Catalysis for Signal-Switchable Photoelectrochemical Biosensing.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01457
Abstract: Polarization of photoactive materials in current photoelectric (PE) systems is difficult to be adjusted, and thus electron-transfer routes of these systems are unchangeable, which limits their performance in photoelectrochemical (PEC) analysis. Herein, we attempted to… read more here.
Keywords: polarization perovskite; polarization; enzyme catalysis; based heterostructures ... See more keywords
Teaching Enzyme Catalysis Using Interactive Molecular Dynamics in Virtual Reality
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Chemical Education"
DOI: 10.1021/acs.jchemed.9b00181
Abstract: The reemergence of virtual reality (VR) in the past few years has led to affordable, high-quality commodity hardware that can offer new ways to teach, communicate, and engage with complex concepts.... read more here.
Keywords: reality; enzyme catalysis; using interactive; virtual reality ... See more keywords
Calculation of Heat Capacity Changes in Enzyme Catalysis and Ligand Binding
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Chemical Theory and Computation"
DOI: 10.1021/acs.jctc.2c00646
Abstract: It has been suggested that heat capacity changes in enzyme catalysis may be the underlying reason for temperature optima that are not related to unfolding of the enzyme. If this were to be a common… read more here.
Keywords: heat capacity; heat; changes enzyme; capacity changes ... See more keywords
A Substrate-Selective Enzyme-Catalysis Assembly Strategy for Oligopeptide Hydrogel-Assisted Combinatorial Protein Delivery.
Sign Up to like & getrecommendations! Published in 2017 at "Nano letters"
DOI: 10.1021/acs.nanolett.7b03371
Abstract: Oligopeptide hydrogels for localized protein delivery have considerable potential to reduce systemic side effects but maximize therapeutic efficacy. Although enzyme catalysis to induce formation of oligopeptide hydrogels has the merits of unique regio- and enantioselectivity… read more here.
Keywords: protein delivery; protease; oligopeptide hydrogel; oligopeptide hydrogels ... See more keywords
Uncovering the Relationship between the Change in Heat Capacity for Enzyme Catalysis and Vibrational Frequency through Isotope Effect Studies
Sign Up to like & getrecommendations! Published in 2018 at "ACS Catalysis"
DOI: 10.1021/acscatal.8b01025
Abstract: Understanding how enzyme catalysis varies with temperature is key to understanding catalysis itself and, ultimately, how to tune temperature optima. Temperature dependence studies inform on the change in heat capacity during the reaction, ΔCP‡, and… read more here.
Keywords: change heat; isotope; catalysis; enzyme catalysis ... See more keywords
Enzyme Catalysis Biomotor Engineering of Neutrophils for Nanodrug Delivery and Cell-Based Thrombolytic Therapy.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.1c08538
Abstract: Utilizing neutrophils (NEs) to target and deliver nanodrugs to inflammation sites has received considerable attention. NEs are involved in the formation and development of thrombosis by transforming into neutrophil extracellular traps (NETs); this indicates that… read more here.
Keywords: system; catalysis biomotor; nanodrug delivery; delivery ... See more keywords
Programming Dissipation Systems by DNA Timer for Temporally Regulating Enzyme Catalysis and Nanostructure Assembly.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c04405
Abstract: Live cells precisely control their temporal pattern in energy dissipative processes such as catalysis and assembly. Here, we demonstrate a DNA-based artificial dissipative nonequilibrium system where the transient state is controlled by the processive digestion… read more here.
Keywords: catalysis; dna timer; catalysis nanostructure; nanostructure assembly ... See more keywords