3'-O-Caged 2'-Deoxynucleoside Triphosphates for Light-Mediated, Enzyme-Catalyzed, Template-Independent DNA Synthesis.
Sign Up to like & getrecommendations! Published in 2017 at "Current protocols in nucleic acid chemistry"
DOI: 10.1002/cpnc.41
Abstract: Synthesis, purification, and characterization of 3'-O-caged 2'-deoxyribonucleoside triphosphates (dNTPs), namely 3'-O-(2-nitrobenzyl)-2'-deoxy ribonucleoside triphosphates (NB-dNTPs) and 3'-O-(4,5-dimethoxy-2-nitrobenzyl)-2'-deoxy ribonucleoside triphosphates (DMNB-dNTPs), are discussed in detail. A total of eight 3'-O-caged dNTPs are synthesized with specific protocols depending… read more here.
Keywords: template independent; caged deoxynucleoside; deoxynucleoside triphosphates; enzyme catalyzed ... See more keywords
Rational enhancement of enzyme-catalyzed enantioselective reaction by construction of recombinant enzymes based on additive strategy
Sign Up to like & getrecommendations! Published in 2019 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-019-02170-1
Abstract: A rational enhancement of kinetic resolution process for producing (S)-N-(2-ethyl-6-methylphenyl) alanine from racemic methyl ester using lipase B from Candida antarctica (CalB) was investigated. With the benefit results that lipase CalB-catalyzed reactions can be effectively… read more here.
Keywords: rational enhancement; catalyzed enantioselective; reaction; enzyme catalyzed ... See more keywords
The physical organic chemistry of glycopyranosyl transfer reactions in solution and enzyme-catalyzed.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2019.08.003
Abstract: Our understanding of the mechanisms of glycopyranosyl transfer that occur in solution, both for the chemical synthesis of complex structures and that for the cleavage of glycosidic bonds has allowed us to design biologically active… read more here.
Keywords: glycopyranosyl transfer; chemistry; physical organic; transfer ... See more keywords
Enzyme-Catalyzed Hydrolysis of Lipids in Immiscible Microdroplets Studied by Contained-Electrospray Ionization.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c02785
Abstract: Enzyme-catalyzed hydrolysis of lipids was monitored directly in immiscible microdroplet environments using contained-electrospray mass spectrometry. Aqueous solution of the lipase enzyme from Pseudomonas cepacia and the chloroform solution of the lipids were sprayed from separate… read more here.
Keywords: hydrolysis lipids; enzyme catalyzed; catalyzed hydrolysis; electrospray ionization ... See more keywords
Combinatorial Enzymatic Catalysis for Bioproduction of Ginsenoside Compound K.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c08773
Abstract: Ginsenoside compound K (CK) is an emerging functional food or pharmaceutical product. To date, there are still challenges to exploring effective catalytic enzymes for enzyme-catalyzed manufacturing processes and establishing enzyme-catalyzed processes. Herein, we identified three… read more here.
Keywords: enzyme catalyzed; ginsenoside; ginsenoside compound; enzymatic catalysis ... See more keywords
Enzyme-Catalyzed Spiroacetal Formation in Polyketide Antibiotic Biosynthesis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c03313
Abstract: A key step in the biosynthesis of numerous polyketides is the stereospecific formation of a spiroacetal (spiroketal). We report here that spiroacetal formation in the biosynthesis of the macrocyclic polyketides ossamycin and oligomycin involves catalysis… read more here.
Keywords: formation polyketide; biosynthesis; enzyme catalyzed; catalyzed spiroacetal ... See more keywords
Enzyme-Catalyzed Intramolecular Enantioselective Hydroalkoxylation.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b01089
Abstract: Hydroalkoxylation is a powerful and efficient method of forming C-O bonds and cyclic ethers in synthetic chemistry. In studying the biosynthesis of the fungal natural product herqueinone, we identified an enzyme that can perform an… read more here.
Keywords: hydroalkoxylation; enantioselective hydroalkoxylation; reaction; enzyme catalyzed ... See more keywords
Symmetry breaking by enzyme-catalyzed epoxide hydrolysis
Sign Up to like & getrecommendations! Published in 2018 at "IUCrJ"
DOI: 10.1107/s2052252518007005
Abstract: Janfalk Carlsson et al. [IUCrJ (2018), 5, 269–282] describe studies on factors determining selective ring opening of methylstyrene oxide stereoisomers by the epoxide hydrolase StEH1. The stereo-differentiating step is selective hydrolysis of an alkylated intermediate… read more here.
Keywords: epoxide hydrolysis; catalyzed epoxide; breaking enzyme; hydrolysis ... See more keywords
Does metabolite channeling accelerate enzyme-catalyzed cascade reactions?
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0172673
Abstract: Metabolite or substrate channeling is a direct transfer of metabolites from one enzyme to the next enzyme in a cascade. Among many potential advantages of substrate channeling, acceleration of the total reaction rate is considered… read more here.
Keywords: channeling accelerate; cascade reactions; enzyme catalyzed; catalyzed cascade ... See more keywords
Enzyme Catalyzed Copolymerization of Lignosulfonates for Hydrophobic Coatings
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2021.697310
Abstract: Enzymatic polymerization of lignin can generate a variety of value-added products concomitantly replacing fossil-based resources. In line with this approach, a laccase from the thermophilic fungus Myceliophthora thermophila (MtL) was used to couple a hydrophobicity… read more here.
Keywords: hydrophobic coatings; lignosulfonates hydrophobic; catalyzed copolymerization; spectroscopy ... See more keywords
Recent Advances in the Enzymatic Synthesis of Polyester
Sign Up to like & getrecommendations! Published in 2022 at "Polymers"
DOI: 10.3390/polym14235059
Abstract: Polyester is a kind of polymer composed of ester bond-linked polybasic acids and polyol. This type of polymer has a wide range of applications in various industries, such as automotive, furniture, coatings, packaging, and biomedical.… read more here.
Keywords: article; catalyzed polymerization; enzyme catalyzed; synthesis ... See more keywords