DNA-crowded enzyme complexes with enhanced activities and stabilities.
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DOI: 10.1039/c7cc07361e
Abstract: We present a robust and simple method to prepare DNA-crowded enzyme complexes by directly assembling long DNA duplexes on the enzyme surface. DNA-crowded enzyme complexes show boosted substrate turnover numbers, and increased stabilities against various… read more here.
Keywords: complexes enhanced; dna crowded; enzyme complexes; crowded enzyme ... See more keywords
CRISPR/Cas-directed programmable assembly of multi-enzyme complexes.
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DOI: 10.1039/d0cc01174f
Abstract: We describe a versatile CRISPR/Cas-based strategy to construct multi-enzyme complexes scaffolded on a DNA template in programmable patterns. Catalytically inactive dCas9 nuclease was used in combination with SpyCatcher-SpyTag chemistry to assemble enzymes in a highly… read more here.
Keywords: multi enzyme; crispr cas; enzyme complexes; cas directed ... See more keywords