Articles with "enzyme immunoassay" as a keyword



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Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application

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Published in 2017 at "Analytical and Bioanalytical Chemistry"

DOI: 10.1007/s00216-017-0606-1

Abstract: AbstractWith their intriguing structures and properties, metallofullerenols have attracted considerable attention in biological and medical applications. Due to the increasing biomedical interest, effective detection methods are important to monitor and control metallofullerenols. However, the detection… read more here.

Keywords: detection; antibody; antigenicity metallofullerenol; metallofullerenol ... See more keywords
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Clinical performance of a novel chemiluminescent enzyme immunoassay for FGF23

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Published in 2021 at "Journal of Bone and Mineral Metabolism"

DOI: 10.1007/s00774-021-01250-1

Abstract: Measurement of fibroblast growth factor 23 (FGF23) has been reported to be clinically useful for the differential diagnosis of chronic hypophosphatemia. However, assays for research use only are available in Japan. Thus, the objective of… read more here.

Keywords: fgf23; chemiluminescent enzyme; fgf23 related; hypophosphatemic rickets ... See more keywords
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Enzyme Immunoassay of Antibodies to Endogenous Bioregulators for Evaluating the Risk of Heart Disease

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Published in 2017 at "Bulletin of Experimental Biology and Medicine"

DOI: 10.1007/s10517-017-3739-5

Abstract: The levels of natural antibodies to β-endorphin, bradykinin, histamine, dopamine, and serotonin were measured in 271 cardiological patients in order to evaluate the severity of their clinical status. The patients were distributed into groups differing… read more here.

Keywords: antibodies endogenous; evaluating risk; immunoassay antibodies; bioregulators evaluating ... See more keywords
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A sensitive chemiluminescence enzyme immunoassay based on molecularly imprinted polymers solid-phase extraction of parathion.

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Published in 2017 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2017.05.008

Abstract: The chemiluminescence enzyme immunoassay (CLEIA) method responds differently to various sample matrices because of the matrix effect. In this work, the CLEIA method was coupled with molecularly imprinted polymers (MIPs) synthesized by precipitation polymerization to… read more here.

Keywords: chemiluminescence enzyme; molecularly imprinted; parathion; imprinted polymers ... See more keywords
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High affinity antibody based on a rationally designed hapten and development of a chemiluminescence enzyme immunoassay for quantification of Alternariol in fruit Juice, maize and flour.

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Published in 2019 at "Food chemistry"

DOI: 10.1016/j.foodchem.2018.12.127

Abstract: Alternariol (AOH), a secondary metabolites of the genus Alternaria, is a genotoxic mycotoxin. Because the previous "Mannich antigen" for AOH readily underwent unstable induction, a new AOH hapten was designed for inducing a high-quality antibody… read more here.

Keywords: antibody; chemiluminescence enzyme; alternariol; juice ... See more keywords
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The immune complex transfer enzyme immunoassay: Mechanism of improved sensitivity compared with conventional sandwich enzyme immunoassay.

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Published in 2018 at "Journal of immunological methods"

DOI: 10.1016/j.jim.2018.05.010

Abstract: Immune complex transfer enzyme immunoassay (ICT-EIA) is one of the technologies which enables ultrasensitive measurements of protein biomarkers. The ICT-EIA uses two types of beads and sandwich-shaped immune complexes are transferred from the 1st bead… read more here.

Keywords: ict eia; complex transfer; transfer enzyme; immune complex ... See more keywords
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Hapten Synthesis, Antibody Development, and a Highly Sensitive Indirect Competitive Chemiluminescent Enzyme Immunoassay for Detection of Dicamba.

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Published in 2019 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.8b07134

Abstract: Although dicamba has long been one of the most widely used selective herbicides, some U.S. states have banned the sale and use of dicamba because of farmers complaints of drift and damage to nonresistant crops.… read more here.

Keywords: indirect competitive; chemiluminescent enzyme; dicamba; detection dicamba ... See more keywords
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Autoantibodies undetectable by chemiluminescent enzyme immunoassay require extended antigen–antibody reaction time for detection

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Published in 2019 at "British Journal of Dermatology"

DOI: 10.1111/bjd.17121

Abstract: Pemphigus vulgaris (PV) is an autoimmune blistering disease affecting the mucous membranes and the skin.1 PV produces autoantibodies targeting the desmosomal adhesion molecules desmoglein (Dsg)3 and Dsg1.2 To detect these autoantibodies, enzyme-linked immunosorbent assay (ELISA)… read more here.

Keywords: immunoassay require; chemiluminescent enzyme; undetectable chemiluminescent; autoantibodies undetectable ... See more keywords
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Validation of an in-house bovine serum enzyme immunoassay for progesterone measurement.

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Published in 2020 at "Journal of dairy science"

DOI: 10.3168/jds.2020-18824

Abstract: Measuring circulating progesterone (P4) of dairy cows is a key component of many research studies dealing with basic and applied reproduction physiology. The gold standard in dairy cows for the measurement of P4 in serum… read more here.

Keywords: commercial eia; serum; eia; house ... See more keywords
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A Chemiluminescence Enzyme Immunoassay Based on Biotinylated Nanobody and Streptavidin Amplification for Diazinon Sensitive Quantification

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Published in 2023 at "Biosensors"

DOI: 10.3390/bios13060577

Abstract: The advantages of genetic modification and preferable physicochemical qualities make nanobody (Nb) easy to develop a sensitive and stable immunosensor platform. Herein, an indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) based on biotinylated Nb was established… read more here.

Keywords: based biotinylated; quantification; daz; enzyme immunoassay ... See more keywords
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Development of an Automated Chemiluminescent Enzyme Immunoassay for Measuring Thrombopoietin in Human Plasma

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Published in 2022 at "Diagnostics"

DOI: 10.3390/diagnostics12020313

Abstract: Plasma thrombopoietin (TPO) measurements help distinguish between different types of thrombocytopenia but are not feasible in routine clinical practice. We developed a fully automated quantitative chemiluminescent enzyme immunoassay (CLEIA) for measuring TPO (TPO-CLEIA), which is… read more here.

Keywords: thrombopoietin; human plasma; tpo; enzyme immunoassay ... See more keywords