Analytical expressions of amperometric enzyme kinetics pertaining to the substrate concentration using wavelets
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Mathematical Chemistry"
DOI: 10.1007/s10910-019-01017-0
Abstract: In this paper, wavelet-based operational matrix methods have been developed to investigate the approximate solutions for amperometric enzyme kinetics problems. The operational matrices of derivatives have been utilized for solving the nonlinear initial value problems.… read more here.
Keywords: expressions amperometric; kinetics pertaining; analytical expressions; pertaining substrate ... See more keywords
Respiratory properties of fresh black carrot (Dacus Carota L.) based upon non-linear enzyme kinetics approach
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-020-04422-5
Abstract: The present work was undertaken with an objective to evaluate the impact of temperature on respiration rate of fresh black carrot using a non-linear enzyme kinetics approach. Two different models viz. Arrhenius equation (temperature effect)… read more here.
Keywords: fresh black; black carrot; enzyme kinetics; carrot ... See more keywords
IR-Compatible PDMS microfluidic devices for monitoring of enzyme kinetics.
Sign Up to like & getrecommendations! Published in 2018 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.03.006
Abstract: Coupling infrared (IR) spectroscopy to microfluidic devices provides a powerful tool for characterizing complex chemical and biochemical reactions. Examples of microfluidic devices coupled with infrared spectroscopy have been limited, however, largely due to the difficulties… read more here.
Keywords: microfluidic devices; enzyme kinetics; thin pdms; pdms ... See more keywords
One-step enzyme kinetics measurement in 3D printed microfluidics devices based on a high-performance single vibrating sharp-tip mixer.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2021.338677
Abstract: Measuring enzyme kinetics is of great importance to understand many biological processes and improve biosensing and industrial applications. Conventional methods of measuring enzyme kinetics require to prepare a series of solutions with different substrate concentrations… read more here.
Keywords: sharp tip; enzyme kinetics; vibrating sharp; one step ... See more keywords
Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale.
Sign Up to like & getrecommendations! Published in 2020 at "Ecotoxicology and environmental safety"
DOI: 10.1016/j.ecoenv.2020.110215
Abstract: Information on the kinetic characteristics of soil enzymes under long-term arsenic (As) pollution in field soils is scarce. We investigated Michaelis-Menten kinetic properties of four soil enzymes including β-glucosidase (BG), acid phosphatase (ACP), alkaline phosphatase… read more here.
Keywords: field; long term; enzyme kinetics; enzyme ... See more keywords
Thermodynamic Activity-Based Progress Curve Analysis in Enzyme Kinetics.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2017.10.004
Abstract: Macrokinetic Michaelis-Menten models based on thermodynamic activity provide insights into enzyme kinetics because they separate substrate-enzyme from substrate-solvent interactions. Kinetic parameters are estimated from experimental progress curves of enzyme-catalyzed reactions. Three pitfalls are discussed: deviations… read more here.
Keywords: progress; thermodynamic activity; activity based; enzyme kinetics ... See more keywords
CRISPR Enzyme Kinetics for Molecular Diagnostics.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c00525
Abstract: CRISPR-diagnostic assays have gained significant interest in the last few years. This interest has grown rapidly during the current COVID-19 pandemic, where CRISPR-diagnostics have been frontline contenders for rapid testing solutions. This surge in CRISPR-diagnostic… read more here.
Keywords: crispr; crispr enzyme; enzyme kinetics; model ... See more keywords
Benefits of Simulations as Remote Exercises During the COVID-19 Pandemic: An Enzyme Kinetics Case Study
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Chemical Education"
DOI: 10.1021/acs.jchemed.0c00607
Abstract: Teaching practical chemistry skills remotely is not a task that would have been high on the agenda only a few months ago. However, in light of the COVID-19 pandemic, students around the world are having… read more here.
Keywords: chemistry; enzyme kinetics; benefits simulations; simulations remote ... See more keywords
Simultaneous enzymatic activity modulation and rapid determination of enzyme kinetics by highly crystalline graphite dots.
Sign Up to like & getrecommendations! Published in 2017 at "Nanoscale"
DOI: 10.1039/c7nr02285a
Abstract: The research field in enzyme-based biotechnology urgently requires the discovery of new materials and methods with high-performance. Here we report that highly crystalline graphite dots (GDs) can modulate enzyme activities, and simultaneously allow for real-time… read more here.
Keywords: crystalline graphite; modulation; enzyme kinetics; simultaneous enzymatic ... See more keywords
High performance enzyme kinetics of turnover, activation and inhibition for translational drug discovery
Sign Up to like & getrecommendations! Published in 2017 at "Expert Opinion on Drug Discovery"
DOI: 10.1080/17460441.2017.1245721
Abstract: ABSTRACT Introduction: Enzymes are the macromolecular catalysts of many living processes and represent a sizable proportion of all druggable biological targets. Enzymology has been practiced just over a century during which much progress has been… read more here.
Keywords: translational drug; enzyme kinetics; drug; drug discovery ... See more keywords
A simple simulator to teach enzyme kinetics dynamics. Application in a problem-solving exercise
Sign Up to like & getrecommendations! Published in 2017 at "Higher Education Pedagogies"
DOI: 10.1080/23752696.2017.1307693
Abstract: Abstract Enzyme kinetics is an essential part of biochemistry programs, which have been gaining importance in recent years for their applications in biotechnology and biomedicine. The teaching and learning of these issues has been traditionally… read more here.
Keywords: simple simulator; application; problem solving; enzyme kinetics ... See more keywords