A 3D paper microfluidic device for enzyme-linked assays: Application to DNA analysis.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202300143
Abstract: We present a paper microfluidic device capable of conducting enzyme-linked assays: a microfluidic enzyme-linked paper analytical device (μEL-PAD). The system exploits a wash-free sandwich coupling to form beads/analyte/enzyme complexes, which are subsequently added to the… read more here.
Keywords: dna; enzyme linked; enzyme; device ... See more keywords
The agony of choice: Impact of the host animal species on the enzyme‐linked immunosorbent assay performance for host cell protein quantification
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28265
Abstract: Host cell proteins (HCPs) are inevitable process‐related impurities in biotherapeutics commonly monitored by enzyme‐linked immunosorbent assays (ELISAs). Of particular importance for their reliable detection are the anti‐HCP polyclonal antibodies (pAbs), supposed to detect a broad… read more here.
Keywords: enzyme linked; host animal; linked immunosorbent; host ... See more keywords
Enzyme‐linked immunosorbent assays (ELISA) based on thread, paper, and fabric
Sign Up to like & getrecommendations! Published in 2018 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700354
Abstract: This paper describes enzyme‐linked immunosorbent assays (ELISAs) utilizing microfluidic thread/paper‐based analytical devices (μTPAD), microfluidic fabric‐based analytical devices (μFAD), and microfluidic thread‐based analytical devices (μTAD). Here, the quantitative detection of biotinylated goat anti‐mouse IgG (system one)… read more here.
Keywords: system; fmol zone; paper; linked immunosorbent ... See more keywords
Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1016-9_5
Abstract: Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for… read more here.
Keywords: c3a; linked immunosorbent; immunosorbent assay; complement activation ... See more keywords
A novel sandwich enzyme-linked immunosorbent assay with covalently bound monoclonal antibody and gold probe for sensitive and rapid detection of bovine β-lactoglobulin
Sign Up to like & getrecommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-018-1019-5
Abstract: AbstractBovine milk is a recognized allergenic food source with β-lactoglobulin (BLG) as its major allergen. Reliable detection of BLG epitopes can, therefore, be a useful marker for the presence of milk in processed food products,… read more here.
Keywords: detection; blg; antibody; lactoglobulin ... See more keywords
Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans
Sign Up to like & getrecommendations! Published in 2022 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03772-w
Abstract: The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins.… read more here.
Keywords: enzyme linked; flow cytometry; sorbent assay; lectin sorbent ... See more keywords
An enzyme-linked immunosorbent assay for the detection of diacetyl (2,3-butanedione).
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.07.021
Abstract: Diacetyl (2,3-butanedione) is an important metabolic marker of several cancers, as well as an important off-flavour component produced during fermentation. As a small molecule in a complex mixture with many other analytes, existing methods for… read more here.
Keywords: linked immunosorbent; diacetyl; immunosorbent assay; diacetyl butanedione ... See more keywords
Application of docking and active site analysis for enzyme linked biodegradation of textile dyes.
Sign Up to like & getrecommendations! Published in 2019 at "Environmental pollution"
DOI: 10.1016/j.envpol.2019.02.080
Abstract: Growth of textile industries led to production of enormous dye varieties. These textile dyes are largely used, chemically stable and easy to synthesize. But they are recalcitrant and persist as less biodegradable pollutants when discharged… read more here.
Keywords: textile dyes; active site; enzyme; application docking ... See more keywords
An indirect competitive enzyme-linked immunosorbent assay toward the standardization of Pueraria candollei based on its unique isoflavonoid, kwakhurin.
Sign Up to like & getrecommendations! Published in 2019 at "Fitoterapia"
DOI: 10.1016/j.fitote.2018.12.010
Abstract: Kwakhurin (Kwa) is a plant secondary metabolite solely present in Pueraria candollei var. mirifica (P. candollei), which has long been used as a Thai traditional herb for estrogen replacement therapy. Recently, health hazards have arisen… read more here.
Keywords: mab 11f; standardization; linked immunosorbent; immunosorbent assay ... See more keywords
Preparation of monoclonal antibody and development of an enzyme-linked immunosorbent assay for the detection of ceftiofur in animal-derived foods.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2021.114378
Abstract: Ceftiofur (CEF) residues in animal-derived foods are of great concern to farmers, regulatory agencies and consumers. In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was established to quickly monitor CEF residues in… read more here.
Keywords: derived foods; monoclonal antibody; linked immunosorbent; animal derived ... See more keywords
A novel, enzyme-linked immunosorbent assay based on the catalysis of AuNCs@BSA-induced signal amplification for the detection of dibutyl phthalate.
Sign Up to like & getrecommendations! Published in 2018 at "Talanta"
DOI: 10.1016/j.talanta.2017.10.051
Abstract: A novel, competitive, enzyme-linked immunosorbent assay (ELISA) was presented in this paper based on the inhibition of catalysis of AuNCs@BSA triggered by dissolved Ag+ for the detection of dibutyl phthalate (DBP). In the immunoassay system,… read more here.
Keywords: detection; auncs bsa; bsa; linked immunosorbent ... See more keywords