Loop variants of the thermophile Rasamsonia emersonii Cel7A with improved activity against cellulose
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26050
Abstract: Cel7A cellobiohydrolases perform processive hydrolysis on one strand of cellulose, which is threaded through the enzyme's substrate binding tunnel. The tunnel structure results from a groove in the catalytic domain, which is covered by a… read more here.
Keywords: loop variants; cel7a; enzyme substrate; rasamsonia emersonii ... See more keywords
Mapping enzyme-substrate interactions: its potential to study the mechanism of enzymes.
Sign Up to like & getrecommendations! Published in 2020 at "Advances in protein chemistry and structural biology"
DOI: 10.1016/bs.apcsb.2020.06.001
Abstract: With the increase of the need to use more sustainable processes for the industry in our society, the modeling of enzymes has become crucial to fully comprehend their mechanism of action and use this knowledge… read more here.
Keywords: interactions potential; mechanism; enzyme substrate; mapping enzyme ... See more keywords
Enzyme Free Energy Diagrams: Enzyme–Substrate Binding Is Always Spontaneous
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Chemical Education"
DOI: 10.1021/acs.jchemed.0c01370
Abstract: In at least two papers published in this Journal, enzyme free energy diagrams were presented showing enzyme–substrate binding to be nonspontaneous. I present here several different approaches showi... read more here.
Keywords: enzyme substrate; enzyme free; substrate binding; energy diagrams ... See more keywords
Critical issues in molecular recognition: the enzyme-substrate association.
Sign Up to like & getrecommendations! Published in 2021 at "Soft matter"
DOI: 10.1039/d1sm00470k
Abstract: Chemical and shape recognition are the main assembling mechanisms of complex bio-structures. A refined assessment of relevant thermodynamic parameters includes the consideration of a variety of contributions from different molecular motions and electronic interactions. An… read more here.
Keywords: critical issues; enzyme substrate; recognition; molecular recognition ... See more keywords
Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
Sign Up to like & getrecommendations! Published in 2019 at "Bioengineering"
DOI: 10.3390/bioengineering7010005
Abstract: Enzymatic hydrolysis of soybean milk proteins with cysteine protease papain was performed in an advanced bioreactor, operated with batch mode. In soybean milk protein hydrolysis reaction, enzyme and substrate ratio and reaction temperature were varied,… read more here.
Keywords: milk; soybean milk; enzyme substrate; hydrolysis ... See more keywords