Structural enzymology binding studies of the peptide‐substrate‐binding domain of human collagen prolyl 4‐hydroxylase (type‐II): High affinity peptides have a PxGP sequence motif
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3450
Abstract: The peptide‐substrate‐binding (PSB) domain of collagen prolyl 4‐hydroxylase (C‐P4H, an α2β2 tetramer) binds proline‐rich procollagen peptides. This helical domain (the middle domain of the α subunit) has an important role concerning the substrate binding properties… read more here.
Keywords: substrate binding; high affinity; psb; enzymology ... See more keywords
Preparation of Cytolysin A (ClyA) Nanopores.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0806-7_2
Abstract: The ionic currents passing through nanopores can be used to sequence DNA and identify molecules at the single-molecule level. Recently, researchers have started using nanopores for the detection and analysis of proteins, providing a new… read more here.
Keywords: preparation cytolysin; clya nanopores; clya; enzymology ... See more keywords
Nanopore Enzymology to Study Protein Kinases and Their Inhibition by Small Molecules.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0806-7_8
Abstract: Nanopore enzymology is a powerful single-molecule technique for the label-free study of enzymes using engineered protein nanopore sensors. The technique has been applied to protein kinases, where it has enabled the full repertoire of kinase… read more here.
Keywords: protein; inhibition; protein kinases; study ... See more keywords
Micellar Enzymology for Thermal, pH, and Solvent Stability.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6499-4_3
Abstract: This chapter describes methods for enzyme stabilization using micellar solutions. Micellar solutions have been shown to increase the thermal stability, as well as the pH and solvent tolerance of enzymes. This field is traditionally referred… read more here.
Keywords: thermal solvent; solvent stability; enzymology thermal; micellar enzymology ... See more keywords
Nanozymes: an emerging field bridging nanotechnology and enzymology
Sign Up to like & getrecommendations! Published in 2019 at "Science China Life Sciences"
DOI: 10.1007/s11427-019-1557-8
Abstract: School of Future Technology, University of Chinese Academy of Sciences, Beijing 101408, China; CAS Engineering Laboratory for Nanozyme, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;… read more here.
Keywords: nanotechnology; emerging field; field bridging; bridging nanotechnology ... See more keywords
Mechanisms of macromolecular reactions.
Sign Up to like & getrecommendations! Published in 2022 at "History and philosophy of the life sciences"
DOI: 10.1007/s40656-022-00492-0
Abstract: During the past two decades, philosophers of biology have increasingly turned their attention to mechanisms of biological phenomena. Through analyses of mechanistic proposals advanced by biologists, the goal of these philosophers is to understand what… read more here.
Keywords: enzymology; chemistry; reaction; macromolecular reactions ... See more keywords
Platensimycin and platencin: Inspirations for chemistry, biology, enzymology, and medicine
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical Pharmacology"
DOI: 10.1016/j.bcp.2016.11.013
Abstract: &NA; Natural products have served as the main source of drugs and drug leads, and natural products produced by microorganisms are one of the most prevalent sources of clinical antibiotics. Their unparalleled structural and chemical… read more here.
Keywords: medicine; natural products; chemistry; biology ... See more keywords
Two products one catalyst: Emulsifiers and biodiesel production combining enzymology, nanostructured materials engineering and simulation models
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Engineering Journal"
DOI: 10.1016/j.cej.2018.05.048
Abstract: Abstract Crises are the driving force of the inventions that have led man to overcome himself. The challenge that concerns us today is to maintain our lifestyle with the energy expenditure involved. In the present… read more here.
Keywords: production; enzymology nanostructured; two products; nanostructured materials ... See more keywords
Biosynthesis of fatty acid-derived hydrocarbons: perspectives on enzymology and enzyme engineering.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2019.07.005
Abstract: Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels.… read more here.
Keywords: biosynthesis fatty; acid derived; derived hydrocarbons; fatty acid ... See more keywords
Enzymology and biosynthesis of the orsellinic acid derived medicinal meroterpenoids.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2020.11.016
Abstract: The advent of synthetic biology has yielded fruitful studies on orsellinic acid-derived meroterpenoids, which reportedly possess important biological activities. Genomics and transcriptomics have significantly accelerated the discovery of the biosynthetic genes for orsellinic acid-derived fungal… read more here.
Keywords: acid derived; orsellinic acid; enzymology biosynthesis; enzymology ... See more keywords
Deep enzymology studies on DNA methyltransferases reveal novel connections between flanking sequences and enzyme activity.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.167186
Abstract: DNA interacting enzymes recognize their target sequences embedded in variable flanking sequence context. The influence of flanking sequences on enzymatic activities of DNA methyltransferases (DNMTs) can be systematically studied with "deep enzymology" approaches using pools… read more here.
Keywords: flanking sequences; dna; deep enzymology; enzymology ... See more keywords