Enhanced One‐Pot Dual‐CRISPR‐Based Assay Lyophilized on a 3D‐Printed Disc for Field‐Deployable Multiplex Bacteria Detection
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DOI: 10.1002/advs.202509355
Abstract: One‐pot CRISPR‐based detection combining recombinase polymerase amplification (RPA) enables rapid and accurate nucleic acid testing but faces challenges in performance, multiplexing, and field‐ready lyophilization. Here, an enhanced one‐pot, helicase‐assisted RPA (hRPA)‐combined, dual‐CRISPR/uAsCas12a (EOD‐CRISPR) assay is… read more here.
Keywords: detection; one pot; eod crispr; crispr ... See more keywords