Epigenetic editing: Dissecting chromatin function in context
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DOI: 10.1002/bies.202000316
Abstract: How epigenetic mechanisms regulate genome output and response to stimuli is a fundamental question in development and disease. Past decades have made tremendous progress in deciphering the regulatory relationships involved by correlating aggregated (epi)genomics profiles… read more here.
Keywords: chromatin; dissecting chromatin; chromatin function; epigenetic editing ... See more keywords
CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology journal"
DOI: 10.1002/biot.201700217
Abstract: Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/-out, subclonal variation, induced unknown regulatory effects as well as overexpression stress… read more here.
Keywords: gene; epigenetic editing; cho cells; gene expression ... See more keywords
Epigenetic editing: How cutting-edge targeted epigenetic modification might provide novel avenues for autoimmune disease therapy.
Sign Up to like & getrecommendations! Published in 2018 at "Clinical immunology"
DOI: 10.1016/j.clim.2018.02.001
Abstract: Autoimmune diseases are enigmatic and complex, and most been associated with epigenetic changes. Epigenetics describes changes in gene expression related to environmental influences mediated by a variety of effectors that alter the three-dimensional structure of… read more here.
Keywords: edge targeted; cutting edge; targeted epigenetic; autoimmune disease ... See more keywords
Ethics and regulatory considerations for the clinical translation of somatic cell human epigenetic editing
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DOI: 10.1016/j.stemcr.2021.06.004
Abstract: Altering the human epigenome with gene-editing technology in attempt to treat a variety of diseases and conditions seems scientifically feasible. We explore some of the ethical and regulatory issues related to the clinical translation of… read more here.
Keywords: epigenetic editing; ethics regulatory; clinical translation; human epigenetic ... See more keywords
Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics
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DOI: 10.1038/srep42047
Abstract: Enzymes involved in epigenetic processes such as methyltransferases or demethylases are becoming highly utilized for their persistent DNA or histone modifying efficacy. Herein, we have developed an optogenetic toolbox fused to the catalytic domain (CD)… read more here.
Keywords: ascl1 promoter; ascl1 gene; editing ascl1; gene ... See more keywords
Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance
Sign Up to like & getrecommendations! Published in 2023 at "Biomedicines"
DOI: 10.3390/biomedicines11051238
Abstract: As advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA without changing its… read more here.
Keywords: methylation editing; increasing specificity; dna methylation; targeted dna ... See more keywords