Allele-Specific Epigenome Editing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7774-1_6
Abstract: The discovery and adaptation of the CRISPR/Cas system for epigenome editing has allowed for a straightforward design of targeting modules which can direct epigenetic editors to virtually any genomic site. This advancement in DNA-targeting technology… read more here.
Keywords: specific epigenome; epigenome editing; allele specific;
Targeted in vivo epigenome editing in medaka fish
Sign Up to like & getrecommendations! Published in 2017 at "Mechanisms of Development"
DOI: 10.1016/j.mod.2017.04.379
Abstract: between transcription factors and protein partners. For the BioID analysis, TWIST1 was fused to the BirA* biotin ligase that will biotinylate TWIST1 interacting proteins in the native condition and a cellular environment. The biotinylated proteins… read more here.
Keywords: medaka fish; vivo epigenome; targeted vivo; twist1 ... See more keywords
Turning up the Heat with Therapeutic Epigenome Editing.
Sign Up to like & getrecommendations! Published in 2018 at "Cell stem cell"
DOI: 10.1016/j.stem.2017.12.013
Abstract: CRISPR-Cas9 has been touted for therapeutic genome editing, but limitations include inefficient correction of disease-causing mutations and off-target mutagenesis. In the latest issue of Cell, Liao et al. (2017) show that a modified version of CRISPR-Cas9… read more here.
Keywords: heat therapeutic; therapeutic epigenome; turning heat; epigenome editing ... See more keywords
Determinants of heritable gene silencing for KRAB-dCas9 + DNMT3 and Ezh2-dCas9 + DNMT3 hit-and-run epigenome editing
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac123
Abstract: Abstract Precision epigenome editing has gained significant attention as a method to modulate gene expression without altering genetic information. However, a major limiting factor has been that the gene expression changes are often transient, unlike… read more here.
Keywords: ezh2; epigenome editing; dcas9 dnmt3; krab dcas9 ... See more keywords
Inducible epigenome editing probes for the role of histone H3K4 methylation in Arabidopsis heat stress memory
Sign Up to like & getrecommendations! Published in 2022 at "Plant Physiology"
DOI: 10.1093/plphys/kiac113
Abstract: Abstract Histone modifications play a crucial role in the integration of environmental signals to mediate gene expression outcomes. However, genetic and pharmacological interference often causes pleiotropic effects, creating the urgent need for methods that allow… read more here.
Keywords: methylation; histone; h3k4; memory ... See more keywords
Concurrent genome and epigenome editing by CRISPR-mediated sequence replacement
Sign Up to like & getrecommendations! Published in 2019 at "BMC Biology"
DOI: 10.1186/s12915-019-0711-z
Abstract: BackgroundRecent advances in genome editing have facilitated the direct manipulation of not only the genome, but also the epigenome. Genome editing is typically performed by introducing a single CRISPR/Cas9-mediated double-strand break (DSB), followed by non-homologous… read more here.
Keywords: replacement; genome editing; methylation; crispr ... See more keywords
Advances of Epigenetic Biomarkers and Epigenome Editing for Early Diagnosis in Breast Cancer
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23179521
Abstract: Epigenetic modifications are known to regulate cell phenotype during cancer progression, including breast cancer. Unlike genetic alterations, changes in the epigenome are reversible, thus potentially reversed by epi-drugs. Breast cancer, the most common cause of… read more here.
Keywords: breast cancer; epigenetic modifications; advances epigenetic; epigenome editing ... See more keywords
Advances in Plant Epigenome Editing Research and Its Application in Plants
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24043442
Abstract: Plant epistatic regulation is the DNA methylation, non-coding RNA regulation, and histone modification of gene sequences without altering the genome sequence, thus regulating gene expression patterns and the growth process of plants to produce heritable… read more here.
Keywords: application; crispr cas9; plant; epigenome editing ... See more keywords
Toward the Development of Epigenome Editing-Based Therapeutics: Potentials and Challenges
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24054778
Abstract: The advancement in epigenetics research over the past several decades has led to the potential application of epigenome-editing technologies for the treatment of various diseases. In particular, epigenome editing is potentially useful in the treatment… read more here.
Keywords: editing based; development epigenome; toward development; epigenome editing ... See more keywords