An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells
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DOI: 10.1038/s41598-017-02456-y
Abstract: Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful tool to introduce genetic mutations into the hPSCs for loss-of-function studies. Here,… read more here.
Keywords: system; crispr cas9; gene knockout; highly efficient ... See more keywords
Enhancers Improve the AID-Induced Hypermutation in Episomal Vector for Antibody Affinity Maturation in Mammalian Cell Display
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DOI: 10.3390/antib7040042
Abstract: The induction of somatic hypermutation (SHM) in various cell lines by activation-induced cytidine deaminase (AID) has been used in protein-directed selection, especially in antibody affinity maturation. Several antibody affinity maturation systems based on mammalian cells… read more here.
Keywords: antibody; antibody affinity; aid; hypermutation ... See more keywords