The strengths and limitations of using biolayer interferometry to monitor equilibrium titrations of biomolecules
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DOI: 10.1002/pro.3827
Abstract: Every method used to quantify biomolecular interactions has its own strengths and limitations. To quantify protein‐DNA binding affinities, nitrocellulose filter binding assays with 32P‐labeled DNA quantify Kd values from 10−12 to 10−8 M but have… read more here.
Keywords: protein; equilibrium titrations; dna; biolayer interferometry ... See more keywords