Blastocyst development after intracytoplasmic sperm injection of equine oocytes vitrified at the germinal-vesicle stage.
Sign Up to like & getrecommendations! Published in 2017 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2017.02.004
Abstract: We evaluated the meiotic and developmental competence of GV-stage equine oocytes vitrified under different conditions. In a preliminary study, using dimethyl sulfoxide (D), ethylene glycol (EG) and sucrose (S) as cryoprotectants, the maturation rate was… read more here.
Keywords: blastocyst development; equine oocytes; vitrification; blastocyst ... See more keywords
Simulations of osmotic events in vitrification of equine oocytes and porcine embryos
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DOI: 10.1016/j.cryobiol.2018.10.136
Abstract: High concentrations of cryoprotective agents (CPAs) used for vitrification of equine oocytes and porcine embryos are believed to negatively affect developmental competence. Here, osmotic events were simulated using 2P formalism (Kleinhans, Cryobiology 37, 271–289) to… read more here.
Keywords: oocytes porcine; osmotic events; vitrification equine; equine oocytes ... See more keywords
Loading equine oocytes with cryoprotective agents captured with a finite element method model
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DOI: 10.1038/s41598-021-99287-9
Abstract: Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect… read more here.
Keywords: finite element; element method; model; equine oocytes ... See more keywords
An improved vitrification protocol for equine immature oocytes, resulting in a first live foal
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DOI: 10.1111/evj.12747
Abstract: BACKGROUND The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. OBJECTIVES The aim of this study was to compare… read more here.
Keywords: exposure; equine oocytes; oocytes vitrified; vitrification ... See more keywords
Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation
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DOI: 10.3390/ijms24086915
Abstract: In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs the oocyte developmental potential, which may be reflected… read more here.
Keywords: equine oocytes; vitrification; oocytes vitrified; vitrified vitro ... See more keywords