13C-assisted metabolomics analysis reveals the positive correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size in Saccharopolyspora erythraea
Sign Up to like & getrecommendations! Published in 2017 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-017-1792-0
Abstract: Metabolomics analysis is extremely essential to explore the metabolism characteristics of Saccharopolyspora erythraea. The lack of suitable methods for the determination of intracellular metabolites, however, hinders the application of metabolomics analysis for S. erythraea. Acyl-CoAs… read more here.
Keywords: pool; metabolomics analysis; propionyl coa; erythromycin production ... See more keywords
Engineering of an Lrp family regulator SACE_Lrp improves erythromycin production in Saccharopolyspora erythraea.
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DOI: 10.1016/j.ymben.2016.10.012
Abstract: Leucine-responsive regulatory proteins (Lrps) are a group of transcriptional regulators that regulate diverse cellular processes in bacteria and archaea. However, the regulatory role of Lrps in antibiotic biosynthesis remains poorly understood. In this study, we… read more here.
Keywords: sace; sace lrp; erythromycin production; 5387 5386 ... See more keywords
PccD Regulates Branched-Chain Amino Acid Degradation and Exerts a Negative Effect on Erythromycin Production in Saccharopolyspora erythraea
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DOI: 10.1128/aem.00049-18
Abstract: ABSTRACT Branched-chain amino acid (BCAA) degradation is a major source of propionyl coenzyme A (propionyl-CoA), a key precursor of erythromycin biosynthesis in Saccharopolyspora erythraea. In this study, we found that the bkd operon, responsible for… read more here.
Keywords: degradation; bkd; pccd; erythromycin production ... See more keywords