Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9492-2_15
Abstract: The endosomal sorting complex required for transport (ESCRT)-III proteins are known to assemble into filaments that mediate membrane remodeling and fission in various biological processes, including the formation of endosomal multivesicular bodies, viral budding, cytokinesis,… read more here.
Keywords: escrt iii; purification; iii proteins; microscopy ... See more keywords
ESCRT-III accessory proteins regulate fungal development and plant infection in Fusarium graminearum
Sign Up to like & getrecommendations! Published in 2019 at "Current Genetics"
DOI: 10.1007/s00294-019-00949-z
Abstract: Ubiquitinated biosynthetic and surface proteins destined for degradation are sorted into the lysosome/vacuole via the multivesicular body sorting pathway, which depends on the function of ESCRT machinery. Fusarium head blight (FHB) caused by Fusarium graminearum… read more here.
Keywords: escrt iii; fusarium; graminearum; accessory proteins ... See more keywords
Modulation of Membrane Rigidity by the ESCRT-III Complex
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DOI: 10.1016/j.bpj.2016.11.1678
Abstract: The ESCRT-III complexes is an evolutionary conserved membrane scission machinery and it is essential in many cellular processes such as cytokinesis, multivesicular bodies formation, HIV release and nuclear membrane repair. Membrane scission by ESCRT-III is… read more here.
Keywords: escrt iii; modulation membrane; membrane; membrane rigidity ... See more keywords
ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences
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DOI: 10.1016/j.cell.2017.03.020
Abstract: The activation of mixed lineage kinase-like (MLKL) by receptor-interacting protein kinase-3 (RIPK3) results in plasma membrane (PM) disruption and a form of regulated necrosis, called necroptosis. Here, we show that, during necroptosis, MLKL-dependent calcium (Ca2+) influx… read more here.
Keywords: escrt iii; mlkl; cell death; necroptotic cell ... See more keywords
An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission
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DOI: 10.1016/j.cell.2020.07.021
Abstract: Summary The endosomal sorting complex required for transport-III (ESCRT-III) catalyzes membrane fission from within membrane necks, a process that is essential for many cellular functions, from cell division to lysosome degradation and autophagy. How it… read more here.
Keywords: escrt iii; fission; deformation fission; membrane deformation ... See more keywords
ESCRT-III is necessary for the integrity of the nuclear envelope in micronuclei but is aberrant at ruptured micronuclear envelopes generating damage
Sign Up to like & getrecommendations! Published in 2019 at "Oncogenesis"
DOI: 10.1038/s41389-019-0136-0
Abstract: Micronuclei represent the cellular attempt to compartmentalize DNA to maintain genomic integrity threatened by mitotic errors and genotoxic events. Some micronuclei show aberrant nuclear envelopes (NEs) that collapse, generating damaged DNA that can promote complex… read more here.
Keywords: escrt iii; iii; micronuclei; integrity ... See more keywords
ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-05889-9
Abstract: Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the nuclear export of macromolecular complexes, but the molecular basis for this transport remains largely unknown. Here we show that endosomal sorting complex required for transport-III… read more here.
Keywords: escrt iii; iii; nuclear membrane; transport ... See more keywords
The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15205-z
Abstract: Cytokinesis requires the constriction of ESCRT-III filaments on the side of the midbody, where abscission occurs. After ESCRT recruitment at the midbody, it is not known how the ESCRT-III machinery localizes to the abscission site.… read more here.
Keywords: escrt iii; abscission site; alix; abscission ... See more keywords
Anisotropic ESCRT-III architecture governs helical membrane tube formation
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DOI: 10.1038/s41467-020-15327-4
Abstract: ESCRT-III proteins assemble into ubiquitous membrane-remodeling polymers during many cellular processes. Here we describe the structure of helical membrane tubes that are scaffolded by bundled ESCRT-III filaments. Cryo-ET reveals how the shape of the helical… read more here.
Keywords: escrt iii; helical membrane; membrane tube; membrane remodeling ... See more keywords
Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16368-5
Abstract: Endosomal sorting complexes for transport-III (ESCRT-III) assemble in vivo onto membranes with negative Gaussian curvature. How membrane shape influences ESCRT-III polymerization and how ESCRT-III shapes membranes is yet unclear. Human core ESCRT-III proteins, CHMP4B, CHMP2A,… read more here.
Keywords: escrt iii; positively curved; membrane; assemble positively ... See more keywords
Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation
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DOI: 10.1038/s41556-020-0537-5
Abstract: The ESCRT-III membrane fission machinery maintains the integrity of the nuclear envelope. Although primary nuclei resealing takes minutes, micronuclear envelope ruptures seem to be irreversible. Instead, micronuclear ruptures result in catastrophic membrane collapse and are… read more here.
Keywords: escrt iii; chromosome fragmentation; fragmentation; unrestrained escrt ... See more keywords