Study Essential Gene Functions by Plasmid Shuffling.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0868-5_5
Abstract: An essential gene is defined as a gene that cannot be completely removed from the genome. Investigation of an essential gene function is limited because its deletion strain cannot be readily created. Here we describe… read more here.
Keywords: plasmid shuffling; study essential; essential gene; gene ... See more keywords
Peptide-Dependent Growth in Yeast via Fine-Tuned Peptide/GPCR-Activated Essential Gene Expression
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00661
Abstract: Building multicellular microbial consortia that communicate with each other and perform programmed functionalities is the next milestone for synthetic biology. Achieving cell–cell communication within these communities requires programming of the transduction of an extracellular signal… read more here.
Keywords: cell; essential gene; growth; gpcr activated ... See more keywords
Flux-Balance Analysis and Mobile CRISPRi-Guided Deletion of a Conditionally Essential Gene in Shewanella oneidensis MR-1.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00323
Abstract: Carbon-neutral production of valuable bioproducts is critical to sustainable development but remains limited by the slow engineering of photosynthetic organisms. Improving existing synthetic biology tools to engineer model organisms to fix carbon dioxide is one… read more here.
Keywords: essential gene; deletion; conditionally essential; gene ... See more keywords
Putting gene essentiality into context
Sign Up to like & getrecommendations! Published in 2017 at "Nature Reviews Genetics"
DOI: 10.1038/nrg.2017.141
Abstract: been to characterize genes that are essential for cellular or organismal viability. Such sets of genes — which are deemed the most important for survival — have numerous applications, including to design and engineer streamlined… read more here.
Keywords: gene essentiality; essential genes; drug; essential gene ... See more keywords
Adaptation of the tetracycline-repressible system for modulating the expression of essential genes in Cryptococcus neoformans
Sign Up to like & getrecommendations! Published in 2024 at "mSphere"
DOI: 10.1128/msphere.01018-24
Abstract: ABSTRACT The opportunistic human fungal pathogen Cryptococcus neoformans has an enormous impact on human health as the causative agent of cryptococcal meningitis, and there is a dire need to expand our current antifungal arsenal. Essential… read more here.
Keywords: system; tetracycline; essential genes; essential gene ... See more keywords
Essential gene acquisition destabilizes plasmid inheritance
Sign Up to like & getrecommendations! Published in 2021 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1009656
Abstract: Extra-chromosomal genetic elements are important drivers of evolutionary transformations and ecological adaptations in prokaryotes with their evolutionary success often depending on their ‘utility’ to the host. Examples are plasmids encoding antibiotic resistance genes, which are… read more here.
Keywords: essential gene; gene; plasmid inheritance; acquisition ... See more keywords
Identification of discriminant features from stationary pattern of nucleotide bases and their application to essential gene classification
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2023.1154120
Abstract: Introduction: Essential genes are essential for the survival of various species. These genes are a family linked to critical cellular activities for species survival. These genes are coded for proteins that regulate central metabolism, gene… read more here.
Keywords: gene; essential gene; essential genes; gene classification ... See more keywords
A Comprehensive Overview of Online Resources to Identify and Predict Bacterial Essential Genes
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2017.02331
Abstract: Genes critical for the survival or reproduction of an organism in certain circumstances are classified as essential genes. Essential genes play a significant role in deciphering the survival mechanism of life. They may be greatly… read more here.
Keywords: essential genes; predict bacterial; essential gene; gene ... See more keywords
Hammerhead ribozymes directed against mRNA of an essential gene inhibit Escherichia coli growth and enhance tetracycline efficacy
Sign Up to like & getrecommendations! Published in 2025 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2025.1663476
Abstract: Aiming to find novel ways to inhibit bacterial growth, we tested hammerhead ribozymes targeting the mRNA acpP transcript, which encodes the essential acyl carrier protein in Escherichia coli. We engineered ribozymes with varying catalytic cores… read more here.
Keywords: escherichia coli; hammerhead ribozymes; tetracycline efficacy; essential gene ... See more keywords