Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing.
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DOI: 10.1007/978-1-0716-1720-5_13
Abstract: Transposon sequencing (Tn-seq) has greatly accelerated the rate at which gene function can be profiled in microbial organisms. This technique has been applied to the study of the dental caries pathogen Streptococcus mutans where it… read more here.
Keywords: transposon sequencing; analysis; essential genes; identification ... See more keywords
A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential Genes.
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DOI: 10.1007/978-1-0716-1720-5_18
Abstract: Identification of genes essential for structure, function, and survival of a cell type is critical for understanding of the underlying mechanisms. Unfortunately, there is no efficient way to identify such genes. Studies by single-cell RNA… read more here.
Keywords: single cell; cell rna; essential genes; approach ... See more keywords
Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers.
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DOI: 10.1007/978-1-0716-1720-5_19
Abstract: Genetic balancer systems, which allow effective capture and maintenance of lethal mutations stably, play an important role in identifying essential genes. Whole-genome sequencing (WGS) followed by bioinformatics analysis, combined with genetic mapping data analysis, allows… read more here.
Keywords: genes caenorhabditis; caenorhabditis elegans; essential genes; lethal mutations ... See more keywords
Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes.
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DOI: 10.1007/978-1-0716-1720-5_3
Abstract: Target deconvolution of new bioactive agents identified from phenotypic screens remains a challenging task. The discovery of mutations that confer resistance to such agents is regarded as the gold standard proof of target identification. Here,… read more here.
Keywords: mutagenesis; crispr; target; essential genes ... See more keywords
Identification of Essential Genes Using Sequential CRISPR and siRNA Screens.
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DOI: 10.1007/978-1-0716-1720-5_5
Abstract: Genome-wide CRISPR and siRNA screening methodologies are powerful tools that are aptly suited to the discovery of essential genes. In this chapter, we outline our methods to conduct sequential CRISPR and siRNA screens to quickly… read more here.
Keywords: sirna screens; sequential crispr; crispr sirna; essential genes ... See more keywords
A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.
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DOI: 10.1007/978-1-0716-1720-5_8
Abstract: Cyanobacteria, a group of diverse bacteria capable of oxygenic photosynthesis, are excellent models for investigating many important cellular processes, such as photosynthesis, nitrogen fixation, and prokaryotic cell differentiation. They also have great potential to become… read more here.
Keywords: crispr based; method constructing; method; essential genes ... See more keywords
Essentiality, conservation, evolutionary pressure and codon bias in bacterial genomes.
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DOI: 10.1016/j.gene.2018.04.017
Abstract: Essential genes constitute the core of genes which cannot be mutated too much nor lost along the evolutionary history of a species. Natural selection is expected to be stricter on essential genes and on conserved… read more here.
Keywords: essential genes; essentiality conservation; codon bias; conservation ... See more keywords
Putting gene essentiality into context
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DOI: 10.1038/nrg.2017.141
Abstract: been to characterize genes that are essential for cellular or organismal viability. Such sets of genes — which are deemed the most important for survival — have numerous applications, including to design and engineer streamlined… read more here.
Keywords: gene essentiality; essential genes; drug; essential gene ... See more keywords
Identifying novel therapeutic targets in gastric cancer using genome-wide CRISPR-Cas9 screening
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DOI: 10.1038/s41388-022-02177-1
Abstract: Genome-scale CRISPR-Cas9 screening technology is a powerful tool to systematically identify genes essential for cancer cell survival. Herein, TKOv3, a genome-scale CRISPR-Cas9 knock-out library, was screened in the gastric cancer (GC) cells, and relevant validation… read more here.
Keywords: cancer; essential genes; cas9 screening; crispr cas9 ... See more keywords
Synthetic addiction extends the productive life time of engineered Escherichia coli populations
Sign Up to like & getrecommendations! Published in 2018 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1718622115
Abstract: Significance Bioproduction of chemicals offers a sustainable alternative to petrochemical synthesis routes by using genetically engineered microorganisms to convert waste and simple substrates into higher-value products. However, efficient high-yield production commonly introduces a metabolic burden… read more here.
Keywords: addiction; production; escherichia coli; product ... See more keywords
Comprehensive analysis of PNA-based antisense antibiotics targeting various essential genes in uropathogenic Escherichia coli
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DOI: 10.1093/nar/gkac362
Abstract: Antisense peptide nucleic acids (PNAs) that target mRNAs of essential bacterial genes exhibit specific bactericidal effects in several microbial species, but our mechanistic understanding of PNA activity and their target gene spectrum is limited. Here,… read more here.
Keywords: uropathogenic escherichia; pna; escherichia coli; analysis ... See more keywords