Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing.
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DOI: 10.1007/978-1-0716-1720-5_13
Abstract: Transposon sequencing (Tn-seq) has greatly accelerated the rate at which gene function can be profiled in microbial organisms. This technique has been applied to the study of the dental caries pathogen Streptococcus mutans where it… read more here.
Keywords: transposon sequencing; analysis; essential genes; identification ... See more keywords
A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential Genes.
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DOI: 10.1007/978-1-0716-1720-5_18
Abstract: Identification of genes essential for structure, function, and survival of a cell type is critical for understanding of the underlying mechanisms. Unfortunately, there is no efficient way to identify such genes. Studies by single-cell RNA… read more here.
Keywords: single cell; cell rna; essential genes; approach ... See more keywords
Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers.
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DOI: 10.1007/978-1-0716-1720-5_19
Abstract: Genetic balancer systems, which allow effective capture and maintenance of lethal mutations stably, play an important role in identifying essential genes. Whole-genome sequencing (WGS) followed by bioinformatics analysis, combined with genetic mapping data analysis, allows… read more here.
Keywords: genes caenorhabditis; caenorhabditis elegans; essential genes; lethal mutations ... See more keywords
Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1720-5_3
Abstract: Target deconvolution of new bioactive agents identified from phenotypic screens remains a challenging task. The discovery of mutations that confer resistance to such agents is regarded as the gold standard proof of target identification. Here,… read more here.
Keywords: mutagenesis; crispr; target; essential genes ... See more keywords
Identification of Essential Genes Using Sequential CRISPR and siRNA Screens.
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DOI: 10.1007/978-1-0716-1720-5_5
Abstract: Genome-wide CRISPR and siRNA screening methodologies are powerful tools that are aptly suited to the discovery of essential genes. In this chapter, we outline our methods to conduct sequential CRISPR and siRNA screens to quickly… read more here.
Keywords: sirna screens; sequential crispr; crispr sirna; essential genes ... See more keywords
A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.
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DOI: 10.1007/978-1-0716-1720-5_8
Abstract: Cyanobacteria, a group of diverse bacteria capable of oxygenic photosynthesis, are excellent models for investigating many important cellular processes, such as photosynthesis, nitrogen fixation, and prokaryotic cell differentiation. They also have great potential to become… read more here.
Keywords: crispr based; method constructing; method; essential genes ... See more keywords
Stress-Induced Constraint on Expression Noise of Essential Genes in E. coli
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Molecular Evolution"
DOI: 10.1007/s00239-024-10211-x
Abstract: Gene expression is an inherently noisy process that is constrained by natural selection. Yet the condition dependence of constraint on expression noise remains unclear. Here, we address this problem by studying constraint on expression noise… read more here.
Keywords: expression; essential genes; constraint expression; expression noise ... See more keywords
Essentiality, conservation, evolutionary pressure and codon bias in bacterial genomes.
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DOI: 10.1016/j.gene.2018.04.017
Abstract: Essential genes constitute the core of genes which cannot be mutated too much nor lost along the evolutionary history of a species. Natural selection is expected to be stricter on essential genes and on conserved… read more here.
Keywords: essential genes; essentiality conservation; codon bias; conservation ... See more keywords
Putting gene essentiality into context
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DOI: 10.1038/nrg.2017.141
Abstract: been to characterize genes that are essential for cellular or organismal viability. Such sets of genes — which are deemed the most important for survival — have numerous applications, including to design and engineer streamlined… read more here.
Keywords: gene essentiality; essential genes; drug; essential gene ... See more keywords
Identifying novel therapeutic targets in gastric cancer using genome-wide CRISPR-Cas9 screening
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DOI: 10.1038/s41388-022-02177-1
Abstract: Genome-scale CRISPR-Cas9 screening technology is a powerful tool to systematically identify genes essential for cancer cell survival. Herein, TKOv3, a genome-scale CRISPR-Cas9 knock-out library, was screened in the gastric cancer (GC) cells, and relevant validation… read more here.
Keywords: cancer; essential genes; cas9 screening; crispr cas9 ... See more keywords
CRISPR/Cas9 targeting of passenger single nucleotide variants in haploinsufficient or essential genes expands cancer therapy prospects
Sign Up to like & getrecommendations! Published in 2024 at "Scientific Reports"
DOI: 10.1038/s41598-024-58094-8
Abstract: CRISPR/Cas9 technology has effectively targeted cancer-specific oncogenic hotspot mutations or insertion–deletions. However, their limited prevalence in tumors restricts their application. We propose a novel approach targeting passenger single nucleotide variants (SNVs) in haploinsufficient or essential… read more here.
Keywords: cancer; essential genes; targeting passenger; haploinsufficient essential ... See more keywords