Establishment and optimization of NMR-based cell metabonomics study protocols for neonatal Sprague-Dawley rat cardiomyocytes.
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DOI: 10.1016/j.ab.2016.11.009
Abstract: A quenching, harvesting, and extraction protocol was optimized for cardiomyocytes NMR metabonomics analysis in this study. Trypsin treatment and direct scraping cells in acetonitrile were compared for sample harvesting. The results showed trypsin treatment cause… read more here.
Keywords: establishment optimization; cell metabonomics; metabonomics study; optimization nmr ... See more keywords