Deuterium Kinetic Isotope Effects Resolve Low-Temperature Substrate Radical Reaction Pathways and Steps in B12-Dependent Ethanolamine Ammonia-Lyase.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00588
Abstract: The first-order reaction kinetics of the cryotrapped 1,1,2,2-2H4-aminoethanol substrate radical intermediate state in the adenosylcobalamin (B12)-dependent ethanolamine ammonia-lyase (EAL) from Salmonella enterica serovar Typhimurium are measured over the range of 203-225 K by using time-resolved,… read more here.
Keywords: substrate radical; reaction; b12 dependent; ethanolamine ammonia ... See more keywords
Mechanism of Light Induced Radical Pair Formation in Coenzyme B12-Dependent Ethanolamine Ammonia-Lyase
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DOI: 10.1021/acscatal.8b00120
Abstract: Coenzyme B12 (adenosylcobalamin = AdoCbl)-dependent enzymes catalyze complex molecular transformations by employing radical chemistry. The initial step in the native catalytic cycle, upon substrate binding, involves homolytic cleavage of the Co–C bond of AdoCbl to… read more here.
Keywords: coenzyme b12; radical pair; mechanism; ethanolamine ammonia ... See more keywords
Localization and interaction studies of the Salmonella enterica ethanolamine ammonia‐lyase (EutBC), its reactivase (EutA), and the EutT corrinoid adenosyltransferase
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Microbiology"
DOI: 10.1111/mmi.14962
Abstract: Some prokaryotes compartmentalize select metabolic capabilities. Salmonella enterica subspecies enterica serovar Typhimurium LT2 (hereafter S. Typhimurium) catabolizes ethanolamine (EA) within a proteinaceous compartment that we refer to as the ethanolamine utilization (Eut) metabolosome. EA catabolism… read more here.
Keywords: euta; ethanolamine ammonia; euta eutt; ethanolamine ... See more keywords